Conserved sequence related to the 3'-terminal region of retrovirus RNA'S In normal cellular DNAs

Kominami, R.; Tomita, Y.; Connors, E.C.; Hatanaka, M.

Journal of Virology 34(3): 684-692


ISSN/ISBN: 0022-538X
PMID: 6770102
Accession: 005038242

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Nucleotide sequences related to the 3'-terminal portion of retrovirus genomic RNA were detected in the DNA of animals, including humans. DNA complementary to the 400-700 nucleotides from the 3'-terminal end of retrovirus RNA (cDNA3'), which contains the enriched conserved region, was hybridized with DNA from a variety of animal cells [mouse, human, chimpanzee, vervet, pigtailed macaque, squirrel monkey, rabbit, trout, rat, Syrian hamster, chicken]. Under the conditions of annealing in 0.72 M NaCl at C and hydroxyapatite chromatography at C, 20-50% of the radioactivity of cDNA3', prepared from 2 retroviruses, a murine Rauscher virus (RLV) and a baboon virus (M7), annealed with normal cellular DNA of animals, including human tissue. The thermal denaturation profile revealed considerable mismatching between the duplex of the cDNA3' and human DNA. cDNA3' of retroviruses is most homologous to cellular DNA of the host species of origin and is less homologous to cellular DNA of species that are distant in the phylogeny of the host species. The conservation and evolution of nucleotide sequences related to the 3' end of retrovirus genomes in animal DNA, including humans, suggest that the sequences may have important functions.