Continuous hypothermic perfusion of rabbit kidneys

Jacobsen, I.A.

Cryobiology 15(3): 290-301


ISSN/ISBN: 0011-2240
PMID: 361343
Accession: 005043031

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Vascular resistance, organ weight gain, release of lactate dehydrogenase (LDH) and post-transplant function was studied during and after hypothermic perfusion at C of rabbit kidneys with 6 different perfusion fluids. A mixture of dextran and bovine serum albumin (BSA), BSA alone in various concentrations, and human serum albumin were tested as colloids, and the effect of perfusate osmolality was investigated. The dextran-BSA mixture was superior to 4.5 and 6.0% BSA alone in terms of better perfusion characteristics, better post-transplant function and lower LDH release. Perfusion characteristics during perfusion with human serum albumin and subsequent graft function were not different from those observed in experiments with dextran-BSA, but the LDH release was lower. Perfusate osmolality was increased by the addition of glucose or mannitol. Perfusion characteristics during perfusion with the hypertonic perfusates were not different from those observed during isotonic perfusion, but post-transplant function seemed to be better after perfusion with the fluid made hypertonic with glucose but addition of mannitol seemed to be deleterious. A perfusion fluid of extracellular electrolyte composition, containing human serum albumin as a colloid and made hypertonic with glucose, can be used as a vehicle for cryoprotectants during their addition to rabbit kidneys.