+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Contrasting effects of escherichia coli single stranded dna binding protein on synthesis by t 7 dna polymerase and escherichia coli dna polymerase i large fragment evidence that binding protein inhibits trans lesion synthesis by polymerase



Contrasting effects of escherichia coli single stranded dna binding protein on synthesis by t 7 dna polymerase and escherichia coli dna polymerase i large fragment evidence that binding protein inhibits trans lesion synthesis by polymerase



Journal of Biological Chemistry 261(11): 4847-4854



The effect of Escherichia coli single-stranded DNA binding protein (SSB) on DNA synthesis by T7 DNA polymerase and E. coli DNA polymerase I (large fragment) using native or aminofluorene-modified M13 templates was evaluated by in vitro DNA synthesis assays and polyacrylamide gel electrophoresis analysis. The two polymerase enzymes displayed differential responses to the addition of SSB. T7 DNA polymerase, a enzyme required for the replication of the T7 chromosome, was stimulated by the addition of SSB whether native or modified templates were used. On the other hand, E. coli DNA polymerase I was slightly stimulated by the addition of SSB to the native template but substantially inhibited on modified templates. This result suggests that DNA polymerase I may be able to synthesize past an aminofluorene adduct but that the presence of SSB inhibited this trans-lesion synthesis. Polyacrylamide gels of the products of DNA synthesis by polymerase I supported this inference since SSB caused a substantial increase in the accumulation of shorter DNA chains induced by blockage at the aminofluorene adduct sites.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 005044871

Download citation: RISBibTeXText

PMID: 3514604


Related references

Contrasting effects of escherichia coli single stranded dna binding protein on synthesis by t 7 dna polymerase and escherichia coli dna polymerase i proof that polymerase i can bypass aminofluorene adducts in dna. Abstracts of Papers American Chemical Society 190, 1985

Influence of escherichia coli single stranded dna binding protein on dna synthesis by t 7 dna polymerase. Abstracts of Papers American Chemical Society 190, 1985

Interaction between Escherichia coli DNA polymerase IV and single-stranded DNA-binding protein is required for DNA synthesis on SSB-coated DNA. Nucleic Acids Research 40(13): 6039-6048, 2012

Effect of Escherichia coli DNA binding protein on the transcription of single-stranded phage M13 DNA by Escherichia coli RNA polymerase. Biochemical and Biophysical Research Communications 78(1): 343-349, 1977

Differences in the mechanism of stimulation of T7 DNA polymerase by two binding modes of Escherichia coli single-stranded DNA-binding protein. Journal of Biological Chemistry 270(15): 8910-8919, 1995

Mechanism of stimulation of t 7 dna polymerase by escherichia coli single stranded dna binding protein. Federation Proceedings 45(6): 1778, 1986

Mechanism of stimulation of T7 DNA polymerase by Escherichia coli single-stranded DNA binding protein (SSB). Journal of Biological Chemistry 263(32): 17006-17015, 1988

Levels of DNA topoisomerases, single-stranded-DNA-binding protein, and DNA polymerase I in rho+ and rho-15 strains of Escherichia coli. Journal of Bacteriology 171(9): 5183-5186, 1989

Mutations produced by DNA polymerase III holoenzyme of Escherichia coli after in vitro synthesis in the absence of single-strand binding protein. Molecular Microbiology 4(10): 1645-1652, 1990

Supercoiling and escherichia coli single stranded dna binding protein are required for n 4 virion rna polymerase promoter recognition. Journal of Cellular Biochemistry Supplement (10 PART B): 241, 1986

Polymerase-specific differences in the DNA intermediates of frameshift mutagenesis. In vitro synthesis errors of Escherichia coli DNA polymerase I and its large fragment derivative. Journal of Molecular Biology 207(2): 335-353, 1989

Escherichia coli single-stranded DNA-binding protein mediates template recycling during transcription by bacteriophage N4 virion RNA polymerase. Proceedings of the National Academy of Sciences of the United States of America 100(16): 9250-9255, 2003

Escherichia coli single-stranded DNA-binding protein is a supercoiled template-dependent transcriptional activator of N4 virion RNA polymerase. Genes & Development 6(10): 2010-2019, 1992

Effects of escherichia coli ssb protein on the single stranded dna dependent atpase activity of escherichia coli reca protein evidence that ssb protein facilitates the binding of reca protein to regions of secondary structure within single stranded dna. Journal of Molecular Biology 193(1): 97-114, 1987

The bacteriophage N4-coded single-stranded DNA-binding protein (N4SSB) is the transcriptional activator of Escherichia coli RNA polymerase at N4 late promoters. Journal of Molecular Biology 246(4): 461-471, 1995