Conversion of d xylose into xylitol by xylose reductase from candida pelliculosa coupled with the oxidoreductase system of methanogen strain hu
Kitpreechavanich, V.; Hayashi, M.; Nishio, N.; Nagai, S.
Biotechnology Letters 6(10): 651-656
ISSN/ISBN: 0141-5492 DOI: 10.1007/bf00133831
A preliminary test for the enzymatic conversion of D-xylose into xylitol by the intact cells of C. pelliculosa (xylose reductase) coupled with the intact cells of a formate-utilizing methanogen strain HU (hydrogenase and F420-NADP oxidoreductase) was conducted by using H2 as an electron donor of NADP+. In the system, NADP(H) was well regenerated via the methanogen cells and .apprx. 90% conversion of xylose to xylitol (.apprx. 8 g/l) could be achieved at 35.degree. C and pH 7.5 after 24 h incubation.