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Copper uptake by penicillium ochro chloron influence of ph on toxicity and demonstration of energy dependent copper influx using protoplasts



Copper uptake by penicillium ochro chloron influence of ph on toxicity and demonstration of energy dependent copper influx using protoplasts



Journal of General Microbiology 131(8): 1875-1880



The existence of energy-dependent Cu influx was demonstrated in protoplasts of P. ochro-chloron. Protoplasts and mycelium were tolerant of Cu over the pH range 3.0-5.5 but sensitive at pH 6.0. Uptake of Cu was .apprx. 10 times greater at pH 6.0 than in the lower range. At pH 3.0, influx showed saturation kinetics with a half-maximal influx at an external Cu2+ concentration of 390 .mu.M and a maximum influx rate of 22 nmol h-1 (107 cells)-1. Saturation kinetics were not observed at pH 6.0.

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Accession: 005055643

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DOI: 10.1099/00221287-131-8-1875


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