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Coronavirus infectious bronchitis virus virus retaining spike glycopolypeptide s 2 but not s 1 is unable to induce virus neutralizing or hemagglutination inhibiting antibody or induce chicken tracheal protection

Coronavirus infectious bronchitis virus virus retaining spike glycopolypeptide s 2 but not s 1 is unable to induce virus neutralizing or hemagglutination inhibiting antibody or induce chicken tracheal protection

Journal of General Virology 67(7): 1435-1442

Avian infectious bronchitis coronavirus (IBV) inactivated by .beta.-propiolactone induced partial protection of the trachea in up to 40% of chickens following one intramuscular inoculation 4 to 6 weeks prior to challenge. Retention of an intact tracheal ciliated epithelium 4 days after challenge was the criterion of protection. There was no correlation between protection and serum titres of virus-neutralizing (VN) and haemagglutination-inhibiting (HI) antibody, which were maximal at about 4 weeks after inoculation. Virus from which the S1 but not the S2 (spike-anchoring) spike glycopolypeptide had been removed by urea did not induce protection or VN or HI antibody. Four intramuscular inoculations of monomeric S1 induced VN and HI antibody in two and four chickens respectively. These results indicate that VN and HI antibodies are induced primarily by S1, that intact spikes are a major requirement for the induction of protective immunity and that this property is probably associated with S1.

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Accession: 005057654

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