Correlation between streptomycin resistance and symbiotic properties of rhizobium part 1 conversion of spheroplastizing effective rhizobium trifolii strain b 1 to avirulent rods with changed phage and antibiotic patterns after mutation to high level of streptomycin resistance

Zelazna-Kowalska, I.

Acta Microbiologica Polonica 26(3): 233-242


ISSN/ISBN: 0137-1320
Accession: 005059667

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R. trifolii strain B1, which is infective and fixes N2 during symbiosis with clover plants, shows a peculiar property of undergoing morphological changes during growth, i.e., rods are changed into spheroplast-like forms. It also failed to grow at C. Mutation to high level streptomycin resistance (above 1000 .mu.g/ml) caused loss of this property. Simultaneously with the change in streptomycin resistance, other features of this strain were also changed: infectivity for clover plants, and sensitivity to high temperature, phages and antibiotics. Mutation to low level streptomycin resistance did not change the above-mentioned features of the strain B1.