Section 6
Chapter 5,070

Cross-reactive cellular and humoral immunity to carcinogen-induced chicken fibrosarcomas. I. Protective cross-immunity between transplantable tumor lines

Edelman, A.S.; Xue, B.; Sanchez, P.; Thorbecke, G.J.

Journal of Immunology 135(3): 2206-2212


ISSN/ISBN: 0022-1767
PMID: 3926898
Accession: 005069835

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Dimethylbenz[a]anthracene (DMBA)-induced transplantable fibrosarcomas in B2 homozygous chickens (SC line) grow progressively in normal chickens, but are rejected by chickens immunized previously with irradiated tumor cells and Corynebacterium parvum (Propionibacterium acnes). Tumor-immune chickens resist challenge by the immunizing tumor lines as well as by some, but not all, fibrosarcoma lines. The pattern of cross-reactivity between 4 DMBA-induced transplantable tumor lines was examined in detail. Ability to reject a tumor challenge correlated very well (P < 0.001) with the presence of delayed-type hypersensitivity (DTH) to that tumor. Immunization with 1 of 2 of the DMBA-induced lines tested also caused rejection of transplantable tumors developed from methylcholanthrene-induced and benzo(a)pyrene-induced primary fibrosarcomas. Although immunization with tumor caused DTH to chicken embryo fibroblasts (CEF), immunization with CEF failed to cause protective immunity or DTH to tumors. Presence of protective immunity, where tested, also correlated with the ability of spleen cells from immune donors to inhibit tumor growth in Winn tests. Humoral immunity exhibited even greater cross-reactivity than did cellular immunity. Distinct patterns of cross-reactivity were nervertheless observed with respect to the serum antibodies as detected in ELISA [enzyme-linked immunosorbent assay]. Two of these patterns were also observed in several sera from primary tumor-bearing chickens, both including reactivity with CEF. Such reactivity was absent from normal chicken sera.

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