+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Cyclic amp stimulation of membrane phosphorylation and calcium ion activated magnesium ion dependent atpase ec 3.6.1.15 in cardiac sarcoplasmic reticulum



Cyclic amp stimulation of membrane phosphorylation and calcium ion activated magnesium ion dependent atpase ec 3.6.1.15 in cardiac sarcoplasmic reticulum



Biochimica et Biophysica Acta 461(3): 441-459



Ca2+-activated ATPase (EC 3.6.1.15) in canine cardiac sarcoplasmic reticulum was stimulated 50-80% by cyclic[c]AMP. The relationship of this stimulation to cAMP-dependent membrane phosphorylation with phosphoester bands was studied. cAMP stimulation of ATPase activity was specific for Ca2+-activated ATPase and was half-maximal at about 0.1 .mu.M which is similar to the concentration required for half-maximal stimulation of membrane phosphorylation by endogenous cAMP-stimulated protein kinase (EC 2.7.1.37). cAMP stimulation of Ca2+-activated ATPase was Ca dependent and maximal at calculated Ca2+ concentrations of 2.0 .mu.M. cAMP-dependent Ca2+-activated ATPase correlated well with the cAMP-dependent membrane phosphorylation of which 80% was 20,000 MW protein identified by sodium dodecyl sulfate discontinuous polyacrylamide gel electrophoresis. In trypsin-treated microsomes, cAMP did not stimulate Ca2+-activated ATPase or phosphorylation of the 20,000 MW membrane protein. An endogenous Ca stimulated protein kinase (probably phosphorylase b kinase) with an apparent Km for ATP of 0.21-0.32 mM was present and appeared to be involved in the cAMP-dependent phosphorylation of the 20,000 MW protein which was Ca dependent. cGMP did not inhibit any of the stimulatory effects of cAMP. Apparently the cAMP stimulation of Ca2+-activated ATPase in cardiac sarcoplasmic reticulum is mediated by the 20,000 MW phosphoprotein product of a series of kinase reactions similar to those activating phosphorylase b.

(PDF emailed within 1 workday: $29.90)

Accession: 005078385

Download citation: RISBibTeXText


Related references

Regulation of calcium uptake and calcium magnesium atpase by cyclic amp dependent phosphorylation in rat cardiac sarcolemma and sarcoplasmic reticulum. Dumont, J E , P Greengard And G A Robison (Ed ) Advances in Cyclic Nucleotide Research, Vol 14 4th International Conference, Brussels, Belgium, July 22-26, 1980 Xxxi+724p Raven Press: New York, N Y , Usa Illus P691, 1981

Mechanism of the stimulation of calcium ii dependent atpase of cardiac sarcoplasmic reticulum by cyclic amp. Journal of Molecular & Cellular Cardiology 11(SUPPL 3): 34, 1979

Effects of cyclic amp dependent and calmodulin dependent phosphorylation of phospholamban on calcium transport atpase of cardiac sarcoplasmic reticulum. Journal of Molecular & Cellular Cardiology 13(SUPPL 2): 85, 1981

Transient state kinetic studies of calcium dependent atpase and calcium transport by cardiac sarcoplasmic reticulum effect of cyclic amp dependent protein kinase ec 2.7.1.37 catalyzed phosphorylation of phospholamban. Journal of Biological Chemistry 255(5): 1985-1992, 1980

Effects of phospholamban phosphorylation catalyzed by cyclic amp dependent and calmodulin dependent protein kinases on calcium transport atpase of cardiac sarcoplasmic reticulum. Journal of Molecular & Cellular Cardiology 15(5): 335-346, 1983

Phosphorylation of phospholamban by calcium-activated, phospholipid-dependent protein kinase. Stimulation of cardiac sarcoplasmic reticulum calcium uptake. Journal of Biological Chemistry 259(13): 8029-8032, 1984

Transient state kinetic studies of calcium ii dependent atpase of cardiac sarcoplasmic reticulum effect of cyclic amp dependent protein kinase catalyzed phosphorylation of phospholamban. Journal of Molecular & Cellular Cardiology 11(SUPPL 3): 34, 1979

Parallelism between cyclic amp dependent protein kinase catalyzed phospholamban phosphorylation and stimulation of calcium transport in cardiac sarcoplasmic reticulum. American Journal of Cardiology 33(1): 172, 1974

Mechanism of the stimulation of calcium dependent atpase of cardiac sarcoplasmic reticulum by cyclic amp dependent protein kinase role of the 22000 dalton protein. Journal of Biological Chemistry 254(2): 319-326, 1979

Phosphorylation of the calcium ion magnesium ion dependent atpase of the sarcoplasmic reticulum coupled with cation translocation. Journal of Biochemistry 71(6): 1101-1104, 1972

Regulation of calcium ii transport by cyclic amp dependent and calcium calmodulin dependent phosphorylation of cardiac sarcoplasmic reticulum. Biophysical Journal 45(2 PART 2): 6A, 1984

Effect of cyclic amp and calcium ii dependent phosphorylation of phospholamban on calcium ii dependent atpase and calcium transport by cardiac sarcoplasmic. Journal of Molecular & Cellular Cardiology 12(SUPPL 1): 164, 1980

Effect of phospholamban phosphorylation on calcium dependent atpase of solubilized cardiac sarcoplasmic reticulum. Japanese Circulation Journal 47(8): 1021, 1983

Membrane fluidity is not an important physiological regulator of the calcium magnesium dependent atpase of sarcoplasmic reticulum. Journal Of Biological Chemistry3: 8070-8071, 1984

Effect of cyclic amp dependent phosphorylation on calcium affinity of cardiac sarcoplasmic reticulum. Biophysical Journal 41(2 PART 2): 17A, 1983