Cyto genetic effects of anti neoplastic drugs on human peripheral lymphocytes in vitro part 1 influence of adriamycin on nuclear division chromosome damage and cell viability
Bempong, M.A.; Brooks, M.N.
Science of Biology Journal 4(1): 1-12
ISSN/ISBN: 0098-5600 Accession: 005081734
Cultured human lymphocytes, exposed to varying concentrations of adriamycin [an antineoplastic drug] (ADM) at different periods during a 72 h culture schedule, were monitored for the cytostatic, cytotoxic and clastogenic effects of the drug. A comparison of the treated and concurrent control cells showed that ADM significantly inhibited the progression of the cell cycle and that the severity of mitotic depression was dependent on the duration of ADM exposure. The incidence of ADM-induced binucleates decreased with increased exposure to the drug. Time- and dose-response curves indicated that the duration of exposure and the concentration of the drug influenced the cytological behavior of the lymphocytes. Longer exposure time and higher concentrations increased ADM cytoxicity and reduced the incidence of observable cytological anomalies. Analysis of clastogenic effect on ADM revealed that the drug induced more isochromatid breaks than chromatid breaks, although, the incidence of chromatid exchanges exceeded that of chromosome exchanges. On the basis of the data on induction of structural rearrangements, it was suggested that breakage-reunion and incomplete exchange events were the underlying causes of the types and frequencies of fragments and exchanges generated by the treated cells.