Cytokinetic and cytogenetic effect of agricultural chemicals on human lymphoid cells in vitro. II. Organochlorine pesticides

Sobti, R.C.; Krishan, A.; Davies, J.

Archives of Toxicology 52(3): 221-231


ISSN/ISBN: 0340-5761
PMID: 6860144
Accession: 005082424

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Human lymphoid cells of LAZ-007 cell line, incubated with 10-4-10-6 M of 8 different organochlorine pesticides, had dose related cytotoxicity, mitotic depression and cell cycle traverse inhibition. In cultures incubated with 10-4 M concentrations, M1 metaphases were as high as 13% (Dicofol) as compared to < 1% in the controls. The frequency of M3 metaphases in cultures incubated with 10-6 M concentrations ranged from 11% (Chlordane) to 15% (Endosulfan) compared to 17% in control cultures. Statistically significant increase in SCE frequency was seen in cells exposed to Chlordane, Dicofol, Endosulfan and Toxaphene. On metabolic activation with rat liver microsomal S-9 enzymes, Chlordane, Dicofol and Tetradifon induced SCE frequency was higher than that of nonactivated cultures. [Chlorobenzillate, Endrin and Hexachlorophene were also tested.].