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Cyto photometric and auto radiographic studies of dna synthesis in the nurse cells of goldeneyes



Cyto photometric and auto radiographic studies of dna synthesis in the nurse cells of goldeneyes



Tsitologiya 18(4): 438-444



DNA synthesis in nurse cells of the imago polytrophic ovarioles of Chrysopa perla (L.) during oogenesis was studied photometrically and autoradiographically (H3-thymidine incorporation). In the early stages of oogenesis the trophocyte nuclei display different amounts of DNA, ranging within 5 [haploid DNA content]. During previtellogenesis and vitellogenesis, the DNA in the nuclei reaches 135c, and occasionally even 310c. However, no definite ploidy levels were detected in all studied stages of oogenesis. The label locality suggests the synthesis taking place only in regions of the complex nucleoli. The label locality suggests the synthesis taking place only in regions of the complex nucleoli. The lack of polyploidization processes in the trophocytes of Chrysopa, and the augmentation of DNA quantity by means of under-replication of the nucleolar organizer regions are discussed.

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