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Cyto photometric evaluation of g 0 g 1 transition in the cell cycle

Cyto photometric evaluation of g 0 g 1 transition in the cell cycle

Byulleten' Eksperimental'noi Biologii i Meditsiny 95(4): 93-95

Feulgen stained nuclei of PHA[phytohemagglutinin]-stimulated human blood lymphocytes were used for cytophotometric chromatin pattern analysis. Similar distributions of low optical density values indicating the predominance of diffuse chromatin were obtained for G1, S and G2 cells. Condensed chromatin was predominant in G0 and M nuclei. Integral vs. average optical densities scatter plots analyses permitted the distinction of cells undergoing different phases of cell cycle including G0 and G1.

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