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Cyto toxic activity of lymphoid cells from mice immunized with semi allogenic hybrid cells requirement of in vitro lymphoid cells culture for expression of cyto toxicity against a syngeneic chemically induced tumor



Cyto toxic activity of lymphoid cells from mice immunized with semi allogenic hybrid cells requirement of in vitro lymphoid cells culture for expression of cyto toxicity against a syngeneic chemically induced tumor



Cellular Immunology 74(2): 383-393



Semiallogeneic somatic hybrid cells (AB2) derived from fusion of a C57Bl/6 chemically induced fibrosarcoma (MCB6-1) and a fibroblastic cell (A9) of C3H origin were used to immunize C57Bl/6 mice against the parental MCB-1 tumor cells. In vitro immune lymphocytes were directly cytotoxic against AB2 hybrid cells and A9 allogeneic parental cells, but could not lyse the syngeneic MCB6-1 parental tumor cells. After a 4-day culture of these immune lymphocytes, a cytotoxic activity against the syngeneic MBC6-1 tumor cells appeared; expression of such a cytotoxic activity did not require the presence of stimulator cells (mitomycin-treated MCB6-1 tumor cells) during the culture. This cytotoxicity is mediated by T cells, as it was completely abrogated by treatment with anti-Thy 1-2 antiserum and complement. Apparently, a maturation or a differentiation of immune T lymphocytes occurs during in vitro culture, and is necessary for the expression of antitumor cytotoxicity.

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Accession: 005083065

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PMID: 6187476



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