Cyto toxic and mutagenic properties of shale oil byproducts 2. comparison of mutagenic effects at 5 genetic markers induced by retort process water plus near uv light in chinese hamster ovary cells
Chen, D.J.C.; Strniste, G.F.
Environmental Mutagenesis 4(4): 457-468
ISSN/ISBN: 0192-2521 Accession: 005083112
A chinese hamster ovary (CHO) cell line heterozygous at the adenine phosphoribosyl transferase (APRT) locus was used for selection of induced mutants resistant to 8-azaadenine (8AA), 6-thioguanine (6TG), ouabain (OUA), emetine (EMT) and diphtheria toxin (DIP). The expression times necessary for optimizing the number of mutants recovered at the different loci were determined using the known direct acting mutagen, far UV light (FUV), and a complex aqueous organic mixture (shale oil process water) activated with near UV light (NUV). Optimal expression times following treatment with either mutagen was evidently between 2 and 8 days (depending on the genetic marker examined). For CHO cells treated with shale oil process water and subsequently exposed to NUV a linear dose response for mutant induction was observed for all 5 genetic loci. At 10% surviving fraction of cells, between 35- and 130-fold increases above background mutation frequencies were observed for the various markers examined. Among the 5 genetic loci tested, OUAR was the most sensitive marker test. [This study may be related to environmental carcinogenesis.].