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Cyto toxic properties of hydroxylamino misonidazole and amino misonidazole possible metabolic products of misonidazole in hypoxic hela s 3 cells



Cyto toxic properties of hydroxylamino misonidazole and amino misonidazole possible metabolic products of misonidazole in hypoxic hela s 3 cells



Gann 74(5): 693-698



Misonidazole, a derivative of 2-nitroimidazole, has selective cytotoxic activity on hypoxic cells in addition to its radiosensitizing activity. This cytotoxicity is considered to be due to metabolic reduction of the drug. A possible metabolite seems to be hydroxylaminomisonidazole, an intermediate product derived via reduction of the nitro group. Authentic samples of hydroxylamino- and aminomisonidazole (a final reduction product) were synthesized and their cytotoxicity towards HeLa S3 cells were compared with that of misonidazole. After a 3 h exposure to 1 mM hydroxylaminomisonidazole under aerobic and hypoxic conditions, the surviving cell fractions were 0.18 and 0.0056, respectively. This represents a cytotoxicity 5 and 125 times greater, respectively, than that of misonidazole. Under the same conditions, aminomisonidazole showed no apparent cytotoxicity.

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