Cytogenetic effects of gaseous and volatile chemicals on mammalian cells in vitro and in vivo. I. Chromosome aberration tests in cultured mammalian cells

Sofuni, T.; Hayashi, M.; Matsuoka, A.; Sawada, M.; Hatanaka, M.; Ishidate, M.

Eisei Shikenjo Hokoku. Bulletin of National Institute of Hygienic Sciences 1984(102): 77-83

1984


ISSN/ISBN: 0077-4715
PMID: 6532510
Accession: 005085326

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Abstract
The present studies are aimed to detect gaseous and volatile chemicals which induce chromosome aberrations in cultured mammalian cells. Chinese hamster cells were exposed to test chemicals in the gaseous phase, using a gas-exposing system, newly developed at this laboratory. The exposure chamber was equipped with a rotary holder to rotate the cylindrical culture bottles, in which cells growing in a monolayer were exposed directly to gaseous chemicals. The cells were exposed to each test chemical for 1 h, and chromosome preparations were made after incubation for an additional 23 h. Ethylene oxide and methyl acrylate induced chromosome aberrations in 39% and 100% of metaphases at doses of 250 ppm and 190 ppm (the concentration in a gas-generator), respectively, while benzene did not significantly increase the frequencies of chromosome aberrations. Actual concentration of methyl acrylate in the culture bottle monitored by a gas chromatograph was .apprx. 1/3 that in the gas-generator.