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Cytokinesis cell expansion and the potential for cyto kinin autonomous growth in tobacco nicotiana tabacum pith



Cytokinesis cell expansion and the potential for cyto kinin autonomous growth in tobacco nicotiana tabacum pith



Plant Physiology (Rockville) 70(4): 1071-1074



Pith tissue from N. tabacum L. cultivars, Maryland Mammoth or Wisconsin 38, was isolated, free of vascular tissue, and cultured on a medium containing auxin but not cytokinin. Explants from the apical 1 cm of stem, within the pith rib meristem, initiated callus growth with 100% efficiency. Macroscopically visible callus was evident 5 days after the tissue was isolated and the cultures grew persistently in the absence of cytokinin. Heat treatment, sometimes used to initiate cytokinin habituation, was not required. Explants from tissue basipetal to the pith rib meristem declined in the frequency of habituation with increasing distance from the shoot apex. Although pith tissue which was growing, in vivo, was more prone than mature tissue to establish cytokinin-habituated callus, the basipetal decline in habituation frequency extended well beyond the zone of cell expansion. Explants from mature pith 40 cm or more from the shoot apex grew in the absence of cytokinin with 18% frequency, although the response required at least 2 wk of culture. Tissue near the periphery of mature pith was more prone to cytokinin-habituation than tissue from the pith center.

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