Dependence of cyto genetic effect of different concentrations of dipin and fotrin on duration of contact with human lymphocytes
Kirichenko, O.P.; Chebotarev, A.N.
Genetika 12(10): 155-160
1976
ISSN/ISBN: 0016-6758 Accession: 005106802
The effect of dipin at concentrations of 20 and 30 .mu.g/ml and fotrin at concentrations of 25 and 40 .mu.g/ml on chromosomes of human lymphocytes depending on the time of contact was investigated. The experiments were carried out at G0 phase (before adding PGA [poly-L-glutamic acid]) at 37.degree. within 10-20 min. The fixation was made by the 58th h. A mathematical model describes experimental data on the reaction of mutagen-chromosome interaction during the time of exposition. This model suggests the formation of cross-links of DNA chains of molecules of polycentric mutagens. According to this model the following equations were derived: .rho. = 1 - e-2(Kt+.alpha.); X = 2 (eKt+.alpha. -1), where .rho. is a portion of aberrant cells, X is the average number of breaks per cell, e is the time of exposure of mutagens with cells and .alpha. and K are coefficients. Breaks induced by dipin and fotrin are distributed among cells in accordance with negative binominal distribution. The portions of chromatid breaks did not change at any exposure for both mutagens, and remained at the level of 50%. The proportion of symmetrical, asymmetrical and complete and incomplete chromatid exchanges under different experimental conditions did not differ from 1:1. Fotrin induces the formation of exchange aberrations (dicentrics and ring chromosomes) at the G0 phase.