Detection of 41 isolates of necrotic ringspot virus apple mosaic virus and prune dwarf virus in prunus and malus by enzyme linked immuno sorbent assay
Mcmorran, J.P.; Cameron, H.R.
Plant Disease 67(5): 536-538
ISSN/ISBN: 0191-2917 Accession: 005114963
Enzyme-linked immunosorbent assay (ELISA) detected 41 isolates of ilarvirus in Prunus and Malus representing the entire symptomatic and serological range of necrotic ringspot virus (NRSV), apple mosaic virus (ApMV) and prune dwarf virus (PDV). Three isolates reported to be NRSV were not detected. Of 11 antisera used, only NRSV-G, RMV, PDV-B were required to detect all 41 isolates. These 3 antisera, which were commercially available, were combined to detect any of the 41 isolates in singular assays. Results of a comparative bioassay using Chenopodium quinoa and Cucumis sativus are discussed.