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Detection of antibodies against infectious bursal diseases virus a comparison of 3 serological methods



Detection of antibodies against infectious bursal diseases virus a comparison of 3 serological methods



Research in Veterinary Science 38(2): 189-192



Four different oil emulsion infectious bursal disease virus (IBDV) vaccines were inoculated into 4-wk-old specific pathogen-free chickens. At weekly intervals for 5 wk, sera were obtained from the vaccinated birds and from uninoculated control birds and examined for antibodies against IBDV by enzyme-linked immunosorbent assay (ELISA), the quantitative agar gel precipitin (QAGP) test and the virus neutralization (VN) test. There was a highly significant correlation between the mean responses to all tests; the highest correlation (0.818) was between VN and QAGP and the lowest (0.573) between QAGP and ELISA. Generally the ELISA detected positive sera earlier than the VN test which in turn was more sensitive than the QAGP test. The ELISA and QAGP test were less variable, more reproducible and easier to perform than the VN test.

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Accession: 005115486

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