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Detection of antibodies against influenza a viruses h1n1 h3n2 in swine sera by the single radial hemolysis srh and the hemagglutination inhibition hi test



Detection of antibodies against influenza a viruses h1n1 h3n2 in swine sera by the single radial hemolysis srh and the hemagglutination inhibition hi test



Journal of Veterinary Medicine Series B 35(1): 57-63



224 sera collected from slaughtered pigs in Hassia (FRG) were tested for antibodies against 4 influenza-A-virus strains (subtypes H1N1 and H3N2) of human and swine origin using the single radial haemolysis technique (SRH) and the haemagglutination inhibition test (HI). With both methods corresponding positive and negative results were gained in ca. 90% of the sera tested. However, the SRH resulted in about 5% more positive reactions than the HI. Divergent results were observed mainly in sera with low antibody levels. The SRH, which is less affected by nonspecific inhibitors and isoagglutinines, seems to be superior to the HI and suitable for screening large numbers of serum samples. The results also offer first informations of the distribution of influenza-A-virus infections in pigs in the country of Hassia.

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Accession: 005115487

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