Detection of filarial antigen in urine by sandwich elisa enzyme linked immunosorbent assay and its use in diagnosis
Malhotra, A.; Reddy, M.V.R.; Naidu, J.N.; Harinath, B.C.
Indian Journal of Medical Research 81(1): 123-128
ISSN/ISBN: 0019-5340 Accession: 005116849
Filarial antigen was detected in the concentrated urine samples of patients with microfilaremia by sandwich ELISA. Double antibody sandwich ELISA using anti-rabbit filarial antigen Ig fraction was more sensitive than using single antibody and neat urine sample could be used for detection of antigen. Urinary filarial antigen was subsequently used in indirect ELISA for the detection of filarial antibody in the sera of filarial patients. Thirty-six of 42 patients with microfilaremia, 17 of 21 with clinical filariasis, 3 of 21 endemic normals, 2 of 7 nonfilarial helminth-infected nonendemics and none of the 6 nonendemic normal sera showed the presence of filarial antibody.