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Detection of high fructose corn syrup in apple juice by mass spectrometric carbon 13 carbon 12 analysis collaborative study

Doner, L.W.; Phillips, J.G.

Journal - Association of Official Analytical Chemists 64(1): 85-90

1981


ISSN/ISBN: 0004-5756
Accession: 005117225

The addition of high fructose corn syrups (HFCS) to apple juice can be detected by mass spectrometric determination of 13C/12C ratios. Forty-one pure apple juice samples, representing 18 varieties, gave a mean value for .delta.13C of -25.3.permill. (parts per thousand), while 4 HFCS averaged -9.7.permill. In a collaborative study, 1 pure apple juice and 4 apple juice-HFCS mixtures containing from 25-70% apple juice were properly classified by 6 laboratories. Samples with .delta.13C values less negative than -20.2.permill., 4 SD from the mean of pure juices can be classified with a high degree of confidence as adulterated. Because of the range of values for pure apple juices, other interpretations of .delta.13C data on suspect samples are meaningless, i.e., samples with values more negative than -20.2.permill. must be considered unadulterated with HFCS. The 13C/12C mass spectrometric method for corn syrup products, 31.150-31.153, was adopted official first action for detecting HFCS in apple juice.

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