Detection of prunus necrotic ringspot virus and prune dwarf virus in peach prunus persica by enzyme linked immuno sorbent assay
Patrakosol, P.; Yanase, H.
Bulletin of the Fruit Tree Research Station Series A (Yatabe) (9): 165-176
Prunus necrotic ringspot virus (NRSV) and prune dwarf virus (PDV) were detected by the technique of enzyme-linked immunosorbent assay (ELISA) in purified virus preparations at concentrations as low as 8 ng/ml and in crude sap of infected leaf tissue from herbaceous hosts or peach diluted to 1:1600 for NRSV and 1:12,800 for PDV. Infectivity tests using cucumber was less sensitive than ELISA. Indexing peach trees for NRSV and PDV by ELISA can be done anytime of the year with dormant buds or bark when flowers and leaves are not available. Because a small amount of leaf or bark tissue is enough for detection of the virus by ELISA, shoot tips obtained from heat-treated young peach trees and top grafted on seedlings can be indexed for NRSV much earlier by ELISA than by the Shirofugen test. The same indexing results were obtained by both indexing methods. The use of ELISA as a routine indexing method for the recovery of NRSV in heat-treated materials is supported by these results.