Detection of rubella hem agglutination inhibition and virus specific immuno globulin m antibody using trypsin treated human group o erythrocytes in the hem agglutination inhibition test

Al-Nakib, W.; Lilley, H.

Journal of Clinical Pathology 31(8): 730-734

1978


ISSN/ISBN: 0021-9746
Accession: 005118789

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Abstract
The modification of the standard rubella HAI test using trypsin-treated human group O erythrocytes instead of chick erythrocytes was evaluated. Of 816 samples tested by both methods, the titers of 807 (98.9%) sera were in close agreement within an acceptable 2-fold difference. Trypsin-treated human group O erythrocytes usually provided titers that were 2-fold higher than those obtained with chick erythrocytes. In general, a very good correlation between the 2 methods was obtained. Data are presented that emphasize the importance of trypsin treatment of human group O erythrocytes before use in the HAI method. Trypsin-treated human group O erythrocytes can be stored for periods of up to 30 days and used in the HAI test without any appreciable loss of sensitivity or specificity. Chick erythrocytes were replaced with trypsin-treated human group O erythrocytes in the sucrose density gradient/HAI method used for the detection of rubella virus-specific IgM and it was a satisfactory method. Trypsin-treated human group O erythrocytes should replace chick erythrocytes in the standard rubella HAI test since the former provided not only a more sensitive, more economic and less time-consuming method, but also a technique as specific as that using chick erythrocytes.