EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Detection of scrapie associated fibril proteins using anti scrapie associated fibril antibody in non purified tissue preparations



Detection of scrapie associated fibril proteins using anti scrapie associated fibril antibody in non purified tissue preparations



Journal of General Virology 67(4): 671-682



Antisera raised to scrapie-associated fibril (SAF) proteins were used to detect scrapie-specific polypeptides in three different non-purified brain preparations: a synaptosomal-mitochondrial fraction, 20% brain homogenate and 20% brain homogenate extracted with Sarkosyl. The concentration of SAF proteins in the preparations was greater than the quantity of SAF as detected by negative stain electron microscopy. This suggests that not all of the protein exists in the form of SAF. An immunologically reactive 33K to 35K protein was detected in both normal and scrapie brain preparations. This protein was susceptible to complete proteinase K (PK) digestion in normal brain preparations and it is suggested that scrapie infection is responsible for post-translational modifications which confer PK resistance in scrapie preparations. These modifications may also play a role in the antigenic differences seen in a variety of scrapie agents. SAF-specific proteins were also detected in the spinal cords and spleens from scrapie-affected animals. Detergent extraction of material followed by PK treatment and Western blot analysis is a highly specific and sensitive method for the detection of SAF proteins. This procedure could be applied to human neurological diseases of unknown aetiology.

(PDF emailed within 1 workday: $29.90)

Accession: 005118832

Download citation: RISBibTeXText



Related references

Detection of scrapie-associated fibril (SAF) proteins using anti-SAF antibody in non-purified tissue preparations. Journal of General Virology 67: 671-681, 1986

Scrapie associated fibril detection from formaldehyde fixed brain tissue in natural cases of ovine scrapie. Research in Veterinary Science 64(1): 41-44, 1998

The reproducibility of scrapie-associated fibril and PrPSc detection methods after long-term cold storage of natural ovine scrapie-affected brain tissue. Journal of Comparative Pathology 120(4): 357-368, 1999

Reproducibility of scrapie-associated fibril and PrPSc detection methods after long-term cold storage of natural ovine scrapie-affected brain tissue. Research in Veterinary Science 68(Supplement A): 19, June, 2000

Scrapie Replication of scrapie agent and the formation of scrapie associated fibril protein in the course of scrapie infection. 1988

Fibril-like structures in preparations of scrapie prions purified from hamster brain. Proceedings meeting Electron Microscopy Society of America: 1st) 802-803, 1983

Antiserum to scrapie-associated fibril protein cross-reacts with Spiroplasma mirum fibril proteins. Journal of Clinical Microbiology 25(12): 2430-2431, 1987

Comparison of scrapie associated fibril detection and histology in the diagnosis of natural sheep scrapie. Veterinary Record 121(25-26): 591-591, 1987

Comparison of scrapie-associated fibril detection and Western immunoblotting for the diagnosis of natural ovine scrapie. Journal of Comparative Pathology 118(1): 41-49, 1998

Western blot detection of scrapie-associated fibril protein in tissues outside the central nervous system from preclinical scrapie-infected mice. Journal of General Virology 69: 955-960, 1988

Mouse polyclonal and monoclonal antibody to scrapie-associated fibril proteins. Journal of Virology 61(12): 3688-3693, 1987

Molecular pathology of scrapie-associated fibril protein (PrP) in mouse brain affected by the ME7 strain of scrapie. European Journal of Biochemistry 172(2): 271-277, 1988

A rapid and efficient method to enrich scrapie associated fibril protein from scrapie brains of hamsters/. Bioscience Reports 4(2): 165-170, 1984

Restriction fragment length polymorphisms of the scrapie-associated fibril protein (PrP) gene and their association with susceptibility to natural scrapie in British sheep. Journal of General Virology 72: 1287-1292, 1991