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Detections of abnormal antigen in a bovine t cell lymphoma cell line and antibodies to the antigen in sera from cattle affected with sporadic types of bovine leukosis



Detections of abnormal antigen in a bovine t cell lymphoma cell line and antibodies to the antigen in sera from cattle affected with sporadic types of bovine leukosis



Bulletin of the Nippon Veterinary & Animal Science University (33): 28-35



A new tumor cell antigen was demonstrated in most cell membrane and cytoplasm of the long cultured T-cell line BTL-C33,4; it derived from a cattle with calf type of leukosis by indirect immunofluorescence assay (IFA) using serum (titer 1:64) of the same cattle with calf type of leukosis in which antibodies to bovine leukemia virus (BLV) was negative. The tumor cell antigen was detected only in several percent of the non-cultured original tumor cells from the leukosis cattle of calf type by IFA using the same serum. The antigen detected in the BTL-C3 cells was tentatively designated BTLA (BTL cell-associate antigen). Antibodies against the BTLA were found in sera (1:10 to 1:64 dilutons) of 6 of 7 cattes with calf type of leukosis. The high antibody activities were also detected in 3 of 3 cattles with thymic type and 1 cattle with skin type of T-cell lymphoma as well as the cases of calf type. The BLV antibodies were all negative in sera of the anti-BTLA positive sporadic leukosis cattles. The anti-BTLA was negative in 18 of 20 cattles with adult type of leukosis except for very weak positive cases (1:10 dilution) in which the BLV antibodies were all positive, and were all negative in 8 cases of healthy cattles. On EM analyses of the BTL-C3 cells, a number of mature virus particles were detected in the extracellular spaces and the intracellular vacuoles and protoplasm of the continuously long cultured lymphoma cells, which were constituted with envelope (105 to 185 nm), capside (65 to 100 nm) and core (50 to 75 nm). The virion shape, the presence of the intracellular mature virus particles, and the nucleocapsid-like particles in nucleus suggested that the virus was herpesvirus-like particles and not type C particles of the BLV.

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Accession: 005119734

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