EurekaMag.com logo
+ Site Statistics
References:
47,893,527
Abstracts:
28,296,643
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Determination of 3 different pools of reduced 1 carbon substituted folates 3. reversed phase high performance liquid chromatography of the azo dye derivatives of p aminobenzoyl poly gamma glutamates and its application to the study of unlabeled endogenous pteroyl poly glutamates of rat liver


, : Determination of 3 different pools of reduced 1 carbon substituted folates 3. reversed phase high performance liquid chromatography of the azo dye derivatives of p aminobenzoyl poly gamma glutamates and its application to the study of unlabeled endogenous pteroyl poly glutamates of rat liver. Analytical Biochemistry 120(2): 323-329

A new reversed-phase high-performance liquid chromatographic (HPLC) method is described for the separation and quantitation of picomole amounts of the azo dye derivatives of p-aminobenzoylpoly-.gamma.-glutamates. In conjunction with previously described procedures for the differential cleavage of 1-carbon-substituted, reduced folates, this HPLC method provides a rapid, sensitive, and reproducible approach to the quantitation and chain-length determination of 3 pools of unlabeled endogenous pteroylglutamates. Analysis of rat liver (n = 9) yielded the following results (.hivin.x .+-. SE): total folates 14.5 .+-. 1.0 nmol/g; folates of pool 1 (5,10-methylenetetrahydro- and unsubstituted tetra- and dihydrofolates) 2.65 .+-. 0.74 nmol/g; folates of pool 2 (5-methyltetrahydrofolates) 5.30 .+-. 0.36 nmol/g; and folates of pool 3 (5,10-methenyltetrahydro-, 10-formyltetrahydro-, 5-formyltetrahydro-, and 5-formiminotetrahydrofolates) 6.40 .+-. 1.60 nmol/g. Most of the folates of rat liver occur as penta- (7.60 .+-. 0.69 nmol/g) and hexaglutamates (6.00 .+-. 0.29 nmol/g). In pool 3 the hexaglutamates predominate. Folate patterns based on the amount of radioactive label incorporated after a pulse dose of [3H]folic acid after at all times from the true steady-state pattern of unlabeled endogenous folates.


Accession: 005120653

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Eto, I.; Krumdieck, C.L., 1982: Determination of three different pools of reduced one-carbon-substituted folates. III. Reversed-phase high-performance liquid chromatography of the azo dye derivatives of p-aminobenzoylpoly-gamma-glutamates and its application to the study of unlabeled endogenous pteroylpolyglutamates of rat liver. Analytical Biochemistry 120(2): 323-329

Eto, I.; Krumdieck, CL., 1982: Determination of three different pools of reduced one-carbon-substituted folates. 3. Reversed-phase high-performance liquid chromatography of the azo dye derivatives of p-aminobenzoylpoly--glutamates and its application to the study of unlabeled endogenous pteropolyglutamates of rat liver. A new reversed-phase high-performance liquid chromatographic (HPLC) method is described for the separation and quantitation of picomole amounts of the azo dye derivatives of p-aminobenzoylpoly-.gamma.-glutamates. In conjunction with previously des...

Eto, I.; Krumdieck, C.L., 1981: Determination of 3 different pools of reduced 1 carbon substituted folates 2. quantitation and chain length determination of the pteroyl poly glutamates of rat liver. A procedure is described for the chemical quantitation of 3 different pools of 1-carbon-substituted, reduced folates in rat liver with the simultaneous determination of their corresponding poly-.gamma.-glutamyl chain lengths. Pool 1 is composed of...

Dolnick, B.J.; Cheng, Y.C., 1978: Human thymidylate synthetase part 2 derivatives of pteroyl mono glutamates and pteroyl poly glutamates as substrates and inhibitors/. The interaction of purified human thymidylate synthetase [EC 2.1.1.6] with various folate analogs, as substrates and inhibitors, was investigated. 5,10-Methylenetetrahydropteroylpentaglutamate had a lower Km (2.2 .+-. 0.1 .mu.M) and 2 times higher...

Stout, R.W.; Cashmore, A.R.; Coward, J.K.; Horvath, C.G.; Bertino, J.R., 1976: Separation of substituted pteroyl monoglutamates and pteroyl oligo-gamma-L-glutamates by high pressure liquid chromatography. Analytical Biochemistry 71(1): 119-124

Leslie G.I.; Baugh C.M., 1974: The uptake of pteroyl carbon 14 glutamic acid into rat liver and its incorporation into the natural pteroyl poly gamma glutamates of that organ. Biochemistry 13(24): 4957-4961

Thompson, R.; Leichter, J.; Cornwell, P.E.; Krumdieck, C.L., 1977: Alterations in the chain length of pteroyl poly gamma glutamates and in the activity of pteroyl poly gamma glutamate hydrolase in response to changes in the steady state of 1 carbon metabolism. The effect of a high protein diet and of limiting dietary methionine and choline on the relative proportions of the various pteroylpoly-.gamma.-glutamates, conjugase activity and folate content in liver of male Japanese quail was determined. After...

Foo S.K.; Cichowicz D.J.; Shane B., 1980: Cleavage of naturally occurring folates to unsubstituted p aminobenzoyl poly gamma glutamates. A method is described for the quantitative conversion of all naturally occurring folates to unsubstituted p-aminobenzoylpoly-.gamma.-glutamates. The procedure involves acid treatment in the presence of mercaptoethanol to convert 5-formyl- and 10-f...

Cashmore, A.R.; Dreyer, R.N.; Horváth, C.; Knipe, J.O.; Coward, J.K.; Bertino, J.R., 1980: Separation of pteroyl-oligo-gamma-L-glutamates by high-performance liquid-chromatography. Methods in Enzymology 66: 459-468

Leslie, G.I.; Baugh, C.M., 1974: The uptake of pteroyl(14-C)glutamic acid into rat liver and its incorporation into the natural pteroyl poly-gamma-glutamates of that organ. Biochemistry 13(24): 4957-4961