Determination of chlorophyll in marine waters intercomparison of a rapid high performance liquid chromatographic method with full high performance liquid chromatographic spectrophotometric and fluorometric methods

Murray, A.P.; Gibbs, C.F.; Longmore, A.R.; Flett, D.J.

Marine Chemistry 19(3): 211-228


ISSN/ISBN: 0304-4203
Accession: 005122116

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A statistical comparison was made between chlorophyll measurements made on a suite of marine samples by four methods. The methods were one complete and one rapid high-performance liquid chromatographic (HPLC) method, a fluorometric method and the traditional spectrophotometric method. Generally good agreeement was found, provided that consideration was given to the presence of chlorophyllide a and allomers of chlorophyll a as determined by the HPLC methods. The rapid HPLC method (10 min per sample) is recommended as a convenient and accurate alternative to traditional methods for chlorophylls.