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Determination of mianserin and metabolites in plasma by liquid chromatography with electrochemical detection



Determination of mianserin and metabolites in plasma by liquid chromatography with electrochemical detection



Journal of Pharmaceutical Sciences 71(8): 889-892



A procedure for the determination of mianserin [an antidepressant], desmethylmianserin and 8-hydroxymianserin in plasma at therapeutic concentrations by liquid chromatography with electrochemical detection is described. Following a multiple-step extraction from alkaline plasma into methyl-tert-butyl ether, the reconstituted extract was injected onto a reversed-phase trimethylsilyl-packed column and eluted with an acetate-acetonitrile mobile phase containing an ion-paired reagent. The method provides an absolute recovery of 71-76% and a day-to-day precision of 5.4-9.1% for each compound at 25 ng/ml. The minimum quantifiable level for all 3 compounds was 5 ng/ml (R[relative]SD > 11%), and the detector response was linear up to 500 ng/ml. Fixed-dose steady-state plasma level data for 34 patients are reported.

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Accession: 005124581

Download citation: RISBibTeXText

PMID: 7120090

DOI: 10.1002/jps.2600710812


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