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Determination of the distribution of acetyl cholin esterase in the rat brain radioautographic study following fixation of a tritium labeled organo phosphorus compound with a high specificity for acetyl cholin esterase



Determination of the distribution of acetyl cholin esterase in the rat brain radioautographic study following fixation of a tritium labeled organo phosphorus compound with a high specificity for acetyl cholin esterase



Travaux Scientifiques des Chercheurs du Service de Sante des Armees (4): 217-220



The value of radioautography for studying the distribution of acetylcholinesterase (ACE) in the brain and ACE turnover was studied. The neurotoxic substance used was O-ethyl-5,2-(bis(1-methylethyl)amino)ethylmethyl phosphorothioate (MPT), 3H-labeled. Six male rats were intoxicated using an intracarotid approach and were sacrificed 1, 10, 30, 100, 300 and 1000 min thereafter. The results of radioautography regarding the distribution of ACE were compared with those of histochemistry, and the former was found to be more exact because histochemistry detected butyrylcholinesterases at the same time as ACE. Good correlation between ACE and previous reports on muscarine receptors was also reported, and the distribution of ACE was compared with that of acetylcholine and choline acetyltransferase. Most of the intoxicated zones belonged to the limbic and nigrostriatal systems. Regarding fixation kinetics, the maximum fixation was obtained .apprx. 10 min after intoxication. The fixation was at first asymmetrical because the most strongly marked zones were homolateral to the catheter. After 30 min, the asymmetry disappeared as a result of hte appearance of new zones, but also because of the loss of contrast of initially labeled zones.

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