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Development of an in vitro rooting bioassay using juvenile phase stem cuttings of persea americana mill


Journal of Horticultural Science 63(2): 295-302
Development of an in vitro rooting bioassay using juvenile phase stem cuttings of persea americana mill
A rooting test was developed with shoot cuttings taken from aseptically germinated avocado seed. The rooting required treatments in two steps: (1) three days in a medium containing indole-3-butyric acid (IBA) (25 mg l-1) and (2) four to eight weeks in an auxin-free medium. Rooting was accomplished with both media containing 0.3.times. strength Murashige and Skoog salts, 3% sucrose, 0.4 mg l-1 thiamine hydrochloride, 100 mg l-1 i-inositol, and 0.8% 'TC' agar. The auxin-free medium also contained 1 g l-1 activated charcoal.


Accession: 005132470



Related references

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