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Development of an in vitro rooting bioassay using juvenile phase stem cuttings of persea americana mill



Development of an in vitro rooting bioassay using juvenile phase stem cuttings of persea americana mill



Journal of Horticultural Science 63(2): 295-302



A rooting test was developed with shoot cuttings taken from aseptically germinated avocado seed. The rooting required treatments in two steps: (1) three days in a medium containing indole-3-butyric acid (IBA) (25 mg l-1) and (2) four to eight weeks in an auxin-free medium. Rooting was accomplished with both media containing 0.3.times. strength Murashige and Skoog salts, 3% sucrose, 0.4 mg l-1 thiamine hydrochloride, 100 mg l-1 i-inositol, and 0.8% 'TC' agar. The auxin-free medium also contained 1 g l-1 activated charcoal.

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Accession: 005132470

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Related references

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