EurekaMag.com logo
+ Site Statistics
References:
47,893,527
Abstracts:
28,296,643
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Development of an integrated model for analysis of the kinetics of apolipoprotein B in plasma very low density lipoproteins, intermediate density lipoproteins, and low density lipoproteins


, : Development of an integrated model for analysis of the kinetics of apolipoprotein B in plasma very low density lipoproteins, intermediate density lipoproteins, and low density lipoproteins. Journal of Clinical Investigation 76(2): 575-585

To quantify more precisely the metabolism of apolipoprotein B (apo B) in human beings, an integrated model was developed for the analysis of the isotope kinetics of apo B in very low density lipoproteins (VLDL), intermediate density lipoproteins (IDL), and low density lipoproteins (LDL). The experimental basis for model development was a series of 30 triple-isotope studies in which patients received autologous 131I-VLDL, 125I-IDL, and [3H]glycerol as a precursor of VLDL triglycerides. The currently proposed model contains the following components: (a) a VLDL delipidation cascade that has a variable number of subcompartments, (b) a slowly catabolized pool of VLDL, (c) an IDL compartment consisting of two closely connected subcompartments, one of which is outside the immediate circulation, and (d) a two-compartment subsystem for LDL. Because mass data indicate that not all VLDL were converted to LDL, the model allows for irreversible removal of apo B from VLDL (or IDL) subsystems. It accounts for apparent "direct" input of LDL by postulating an early, rapidly metabolized compartment of VLDL that is converted directly to IDL. The model appears to be consistent with specific activity curves from the current triple-isotope studies and with present concepts of lipoprotein physiology; it also can be used to quantify pathways of lipoprotein apo B transport in normal and abnormal states.


Accession: 005132483

PMID: 4031063

DOI: 10.1172/JCI112009

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Musliner T.A.; Krauss R.M., 1986: Lipolysis products promote the formation of complexes of very low density lipoproteins and low density lipoproteins and the association of apolipoprotein a i with very low density lipoproteins and low density lipoproteins. Clinical Research 34(2): 550A

Falconi C., 1984: Electrophoretic mobility of apolipoprotein b in very low density lipoproteins intermediate density lipoproteins and low density lipoproteins of normal subjects. Bulletin Of Molecular Biology & Medicine: 175-182

Talussot, C.; Ponsin, G., 1994: The transformation of nascent disc shaped high density lipoproteins into mature spherical high density lipoproteins: conformational analysis of apolipoprotein A-I in model intermediate particles. The conformation of apolipoprotein A-I (apo A-I) was studied in reassembled lipoprotein particles containing various proportions of apo A-I, phospholipids and cholesteryl esters or triglycerides, using both theoretical and experimental approaches....

Lee, L.T.; Lefevre, M.; Wong, L.; Roheim, P.S.; Thompson, J.J., 1987: Gradient acrylamide/agarose gels for electrophoretic separation of intact human very low density lipoproteins, intermediate density lipoproteins, lipoprotein a, and low density lipoproteins. An exponential gradient gel with 0-10% acrylamide and 0.5% agarose was developed for electrophoresis of intact high molecular weight lipoproteins. This system resolves very low density lipoproteins, intermediate density lipoproteins, lipoprotein a...

Hughes, T.A.; Gaber, A.O.; Montgomery, C.E., 1991: Plasma distribution of cyclosporine within lipoproteins and "in vitro" transfer between very-low-density lipoproteins, low-density lipoproteins, and high-density lipoproteins. Cyclosporine A (CsA) is a very lipophilic, immunosuppressive peptide that is highly bound (greater than 95%) in plasma. Approximately 50% of the drug is bound to lipoproteins and the remainder to erythrocytes. Neither the therapeutic nor the toxic...

Schmitz G.; Assmann G.; Augustin J.; Dirkes Kersting A.; Brennhauesen B.; Karoff C., 1985: Characterization of very low density lipoproteins and intermediate density lipoproteins of normo lipidemic and hyperlipidemic apolipoprotein e 2 homozygotes. Triglyceride-rich lipoproteins derived from 10 normo- and hyperlipidemic apoE-2 homozygotes were analyzed for their composition, .beta.-VLDL [.beta.-very low density lipoprotein] content, and their ability to induce cholesteryl ester storage in ma...

Hollander, W.; Paddock, J.; Colombo, M., 1979: Lipoproteins in human atherosclerotic vessels. I. Biochemical properties of arterial low density lipoproteins, very low density lipoproteins, and high density lipoproteins. Experimental and Molecular Pathology 30(2): 144-171

Lee L.T.; Lefevre M.; Wong L.; Roheim P.S.; Thompson J.J., 1987: Gradient acrylamide agarose gels for electrophoretic separation of intact human very low density lipoproteins intermediate lipoproteins lipoprotein a and low density lipoproteins. An exponential gradient gel with 0-10% acrylamide and 0.5% agarose was developed for electrophoresis of intact high molecular weight lipoproteins. This system resolves very low density lipoproteins, intermediate density lipoproteins, lipoprotein a...

Schmitz, G.; Assmann, G.; Augustin, J.; Dirkes-Kersting, A.; Brennhaüsen, B.; Karoff, C., 1985: Characterization of very low density lipoproteins and intermediate density lipoproteins of normo- and hyperlipidemic apolipoprotein E-2 homozygotes. Triglyceride-rich lipoproteins derived from ten normo- and hyperlipidemic apoE-2 homozygotes were analyzed for their composition, beta-VLDL content, and their ability to induce cholesteryl ester storage in macrophages. In six of these probands apo...

Fan, J.; Ji, Z.S.; Huang, Y.; de Silva, H.; Sanan, D.; Mahley, R.W.; Innerarity, T.L.; Taylor, J.M., 1998: Increased expression of apolipoprotein E in transgenic rabbits results in reduced levels of very low density lipoproteins and an accumulation of low density lipoproteins in plasma. Transgenic rabbits expressing human apo E3 were generated to investigate mechanisms by which apo E modulates plasma lipoprotein metabolism. Compared with nontransgenic littermates expressing approximately 3 mg/dl of endogenous rabbit apo E, male t...