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Development of cell surface saccharides on embryonic pancreatic cells






Journal of Cell Biology 86(1): 96-103

Development of cell surface saccharides on embryonic pancreatic cells

Seven lectin-ferritin conjugates were used as probes for cell surface glycoconjugates to study the pattern of plasmalemmal differentiation of cells in the embryonic rat pancreas from day 15 in utero to the early postpartum stage. Differentiation of plasmalemmal glycoconjugates on acinar, endocrine and centroacinar cell was temporally correlated with development and was unique for each cell type. Expression of adult cell surface saccharide phenotype can be detected on presumptive acinar cells as early as 15 days in utero [as indicated by soybean agglutinin binding], preceding development of intracellular organelles characteristic of mature acinar cells. Maturation of the plasmalemma of acinar cells is reached after intracellular cytodifferentiation is completed, as indicated by appearance of Con [concanavalin] A and fucoselectin [i.e., Ulex europaeus and Lotus tetragonolobus] binding sites at no earlier than day 19 of development. Maturation of the endocrine cell plasmalemma is accompanied by loss (masking) of Ricinus communis agglutinin II receptors. Binding sites for fucose lectins and for soybean agglutinin are absent on endocrine and centroacinar cells at all stages examined. Acinar, centroacinar and endocrine cells apparently develop from a common progenitor cell(s) whose plasmalemmal carbohydrate composition resembles most closely that of the adult centroacinar cell. Appearance of acinar lumina beginning at 17 days in utero is accompanied by differentiation of apical and basolateral plasmalemmal domains of epithelial cells, as indicated by enhanced binding of several lectin-ferritin conjugates to the apical plasmalemmal, a pattern that persists from this stage through adult life. [R. communis agglutinin I and wheat germ agglutinin were also used as conjugates].

Accession: 005132895

PMID: 7419584

DOI: 10.2307/1609076

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