Development of cytochalasin b induced tetra ploid and di ploid tetra ploid mosaic mouse embryos

Tarkowski, A.K.; Witkowska, A.; Opas, J.

Journal of Embryology and Experimental Morphology 41(1): 47-64

1977


ISSN/ISBN: 0022-0752
Accession: 005133148

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Abstract
By subjecting F1(CBA .times. C57/Bl) .times. A eggs to cytochalasin B at the time of 2nd cleavage, tetraploidy was produced in 52% of 2-cell eggs and 35% of 3-cell eggs. There were 2N/4N mosaic embryos produced from 2-, 3- and 4-cell eggs which amounted to 20% of all treated eggs. About 80% of tetraploid embryos developed in vitro into regular blastocysts with half the cell number of control diploids. Postimplantation development of 4N embryos was normal at first but at 7.5 days the embryonic part of the egg-cylinders was underdeveloped and in later development failed to form an embryo. Development of fetal membranes was less affected and in some conceptuses were fully accomplished. Failure of embryonic development appeared due to subnormal activity of primitive streak, resulting in shortage of mesoderm. Postimplantation development of 2N/4N mosaic embryos was normal. Tetraploid cells were practically eliminated from the embryos, though not necessarily from the fetal membranes.