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Development of syncytial raphide crystal idioblasts in the cortex of adventitious roots of vanilla planifolia orchidaceae



Development of syncytial raphide crystal idioblasts in the cortex of adventitious roots of vanilla planifolia orchidaceae



Scanning Electron Microscopy (2): 893-904



The initiation and formation of calcium oxalate raphide crystals and developmental aspects of crystal idioblasts were studied in adventitious roots of V. planifolia L. (Orchidaceae). Root crystal idioblast initials are first recognized by intense fluorescence of the cytoplasm with the acridine orange method; nuclear and nucleolar enlargement gradually ensues. Crystal chambers form in association with an electron dense vacuolar amorphous material and calcium oxalate deposition occurs within these chambers. Vacuolar cables of tubules become associated with the crystals. They apparently are involved with anchorage of the crystals and the orientation of the raphide crystal bundle. Crystalloplastids differentiate lobed regions similar to those seen in other mucilaginous raphide idioblasts, and their development precedes mucilage accumulation. At this time, raphides change from being 4-sided in cross section to 6- and 8-sided. Idioblast maturation is completed as idioblast transverse walls in a file break down to form a syncytium. Bas-relief photographic processing for image enhancement of conventional light and transmission electron micrographs is described.

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Accession: 005135610

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