EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Differences in the behavior of cytoplasmic granules and lipid bodies during human lung mast cell degranulation






Journal of Cell Biology 99(5): 1678-1687

Differences in the behavior of cytoplasmic granules and lipid bodies during human lung mast cell degranulation

A morphometric and autoradiographic approach was used to analyze changes in specific cytoplasmic granules and cytoplasmic lipid bodies associated with human lung mast cell degranulation. Mast cells were dissociated from lung tissue by enzymatic digestion and were then enriched to purities of up to 99% by countercurrent centrifugation elutriation and recovery from columns containing specific antigen bound to Sepharose 6 MB. Degranulation was induced by goat anti-IgE. At various intervals after stimulation, parallel aliquots of cells were recovered for determination of histamine release or were fixed for transmission electron microscopy. Lipid bodies, electron-dense structures that lack unit membranes, were present in both control and stimulated mast cells. Autoradiographic analysis showed that lipid bodies represented the major repository of 3H-label derived from [3H] arachidonic acid taken up from the external milieu. The specific cytoplasmic granules contained no detectable 3H-label. Lipid bodies occurred in intimate association with degranulation channels during mast cell activation, but the total volume of lipid bodies did not change during the 20 min after stimulation with anti-IgE. This result stands in striking contrast to the behavior of specific cytoplasmic granules, the great majority of which (77% according to aggregate volume) exhibited ultrastructural alterations during the first 20 min of mast cell activation. Mast cell cytoplasmic granules and cytoplasmic lipid bodies are distinct organelles that differ in ultrastructure, biochemistry, and behavior during mast cell activation.

Accession: 005151096

PMID: 6436254

DOI: 10.2307/1611102

Download PDF Full Text: Differences in the behavior of cytoplasmic granules and lipid bodies during human lung mast cell degranulation



Related references

Differences in the volume distributions of human lung mast cell granules and lipid bodies: evidence that the size of these organelles is regulated by distinct mechanisms. Journal of Cell Biology 100(5): 1488-1492, 1985

Human lung mast cell and alveolar macrophage cytoplasmic lipid bodies contain arachidonic acid and prostaglandin endoperoxide synthase , the substrate and enzyme necessary for prostaglandin production. International Archives of Allergy & Immunology 99(2-4): 208-217, 1992

Ultrastructural immunogold localization of prostaglandin endoperoxide synthase cyclooxygenase to non membrane bound cytoplasmic lipid bodies in human lung mast cells alveolar macrophages type ii pneumocytes and neutrophils. Journal of Histochemistry & Cytochemistry 40(6): 759-769, 1992

Mast cell secretory granules and lipid bodies contain the necessary machinery important for the in situ synthesis of proteins. Chemical Immunology and Allergy 85: 252-315, 2005

Element constituents of rodent bulbourethral gland goblet granules, vertebrate mast cell granules and human platelet dense bodies by quantitative X-ray microanalysis on fresh preparations. Acta Histochemica et Cytochemica 27(5): 499-505, 1994

Ultrastructural immunolocalization of basic fibroblast growth factor to lipid bodies and secretory granules in human mast cells. Histochemical Journal 33(7): 397-402, 2002

Ultrastructural immunolocalization of basic fibroblast growth factor to lipid bodies and secretory granules in human mast cells. Histochemical Journal 33(7): 397-402, July, 2001

Human chorionic mast cells with numerous lipid bodies and a mixture of immature and mature granules are present in gastroschisis. Pediatric Pathology 11(4): 673-676, 1991

Cytoplasmic lamellar inclusions resembling tissue mast cell granules in suspected rheumatoid lung disease. American Journal of Clinical Pathology 52(5): 593-598, 1969

Granules, lamellar bodies, and cytoplasmic fibrils in neoplastic mast cells in the dog. Berl. Munch. tierarztl. Wschr, Frankfurter Strasse 94, 63 Giessen, 83: 123-127, 1970