EurekaMag.com logo
+ Translate

Differences in the behavior of luteinizing hormones of various species at the rat gonadal cell receptor site


, : Differences in the behavior of luteinizing hormones of various species at the rat gonadal cell receptor site. Endocrinology 116(2): 597-603

The ability of different LH[luteinizing hormone]-like hormones, such as hCG [human chorionic gonadotropin], PMSG [pregnant mare serum gonadotropin] equine (e) CG, ovine (O) LH, eLH and rat (r) LH, to bind to and stimulate steroidogenesis in 2 types of rat gonadal cells was studied under the same experimental conditions. In both Leydig and granulosa cells, the maximal steroidogenic responses elicited by optimal doses of different LH present during a 2 h incubation were comparable. If the cells were exposed to the different LH for a brief period and then subjected to interference with hormone action by removing the unbound hormone from the medium by washing or adding specific antisera, differences were observed in the amount of steroid produced during subsequent incubation in hormone-free medium. In the case of hCG, either of these procedures carried out at 15 or 30 min of incubation had little inhibitory effect on the amount of steroid produced at 2 h; the latter being similar to that produced by cells incubated in the continued presence of hCG for 2 h. With eCG and rLH, the effect was dramatic in that there was a total inhibition of subsequent steroidogenic response. In cells exposed to eLH and oLH, inhibition of subsequent steroidogenesis due to either removal of the free-hormone or addition of specific antisera at 15 or 30 min was only partial. Although all of the antisera used were equally effective in inhibiting the steroidogenic response to respective gonadotropins when added along with hormones at the beginning of incubation, differences were observed in the degree of inhibition of this response when the same antisera were added at later times of incubation. When antisera were added 60 min after the hormone, the inhibition of steroidogenesis was total (100%) for eCG, partial (10-40%) for eLH and oLH, and totally lacking in cells treated with hCG. Apparently, hCG bound to the receptor probably becomes unavailable for binding to its antibody with time, while in the case of eCG and other LH used, the antibody can still inhibit the biological activity of the hormone. Studies with 125I-labeled hormones further supported the conclusion that hCG differs from all other LH in being most tightly bound and, hence, least dissociable, while eCG and rLH dissociate most readily; oLH and eLH can be placed in between these hormones in the extent of their dissociability.

(PDF same-day service: $19.90)

Accession: 005151097

PMID: 2981670

DOI: 10.1210/endo-116-2-597

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Berenbaum, Sheri, A., 1998: How hormones affect behavioral and neural development: Introduction to the special issue on Gonadal Hormones and Sex Differences in Behavior. As the articles in this special issue demonstrate, gonadal hormones have powerful effects on the development of the brain and behavior in human beings, as in other species. Both androgens and estrogens affect behavior throughout development, from...

Hines, M., 1982: Prenatal gonadal hormones and sex differences in human behavior. Psychological Bulletin 92(1): 56-80

Zimmerberg, B.; Farley, M., J., 1993: Sex differences in anxiety behavior in rats: Role of gonadal hormones. These experiments examined the role of gonadal hormones at both the organizational and activational time periods on sex differences in plus-maze behavior. In the first experiment, adult female Long-Evans rats were found to spend more time on the o...

Zimmerberg, B.; Farley, M.J., 1993: Sex differences in anxiety behavior in rats: role of gonadal hormones. These experiments examined the role of gonadal hormones at both the organizational and activational time periods on sex differences in plus-maze behavior. In the first experiment, adult female Long-Evans rats were found to spend more time on the o...

Beatty W.W., 1979: Gonadal hormones and sex differences in nonreproductive behavior in rodents organizational and activational influences. Hormones & Behavior: 112-163

Blizard D.A., 1983: Sex differences in running wheel behavior in the rat the inductive and activational effects of gonadal hormones. The effect of estradiol benzoate (OB) administration on running-wheel activity was compared in gonadectomized male, female and neonatally androgenized female rats. In the 1st experiment, conducted on 300-400-day-old animals, OB stimulated running-...

Blizard, D.A.; Lippman, H.R.; Chen, J.J., 1975: Sex differences in open-field behavior in the rat: the inductive and activational role of gonadal hormones. Physiology & Behavior 14(5): 601-608

Lonstein, J.S.; Devries, G.J., 1998: Sex differences in parental behavior of virgin prairie voles Role of gonadal hormones and vasopressin. Society for Neuroscience Abstracts 24(1-2): 952

Smith, Y.R.; Zubieta, J.K.; del Carmen, M.G.; Dannals, R.F.; Ravert, H.T.; Zacur, H.A.; Frost, J.J., 1998: Brain opioid receptor measurements by positron emission tomography in normal cycling women: relationship to luteinizing hormone pulsatility and gonadal steroid hormones. The regulation of central mu-opioid receptors in women during the menstrual cycle was explored with positron emission tomography and the selective radiotracer (11C)carfentanil. Ten healthy women were studied twice, during their follicular and lute...

B.V.da; E.H.abovszky; T.K.lamatianos; C.W.C.en; Z.L.posits; I.K.lló, 2008: Oestrogen Receptor and Immunoreactive Cells in the Suprachiasmatic Nucleus of Mice: Distribution, Sex Differences and Regulation by Gonadal Hormones. Journal of Neuroendocrinology 20(11): 1270-1277