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Differences in the cellular location of substances endocytosed by rat liver as observed from the distribution patterns obtained after isopycnic centrifugation in a sucrose gradient


, : Differences in the cellular location of substances endocytosed by rat liver as observed from the distribution patterns obtained after isopycnic centrifugation in a sucrose gradient. Biochemical & Biophysical Research Communications 136(2): 504-509

We have investigated the distribution of several substances endocytosed by rat-liver, after isopycnic centrifugation in a sucrose gradient of the MLP fractions (de Duve, Pressman, Gianetto, Wattiaux and Appelmans (1955) Biochem. J. 63, 604-617) isolated at increasing times after injection. It has been observed that there are changes in the distribution pattern with time depending on whether the substance is taken up by parenchymal or sinusoidal cells. The results suggest that centrifugation experiments can be informative with respect to the cellular location of a molecule endocytosed by the liver.

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Accession: 005151141

PMID: 2423084

DOI: 10.1016/0006-291x(86)90469-9

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