EurekaMag.com logo
+ Translate

Differences in the demonstration of complement c 3 polymorphism in high voltage gel electrophoresis depending on agarose quality investigation of rare complement c 3 variants in behring agarose ags 082


, : Differences in the demonstration of complement c 3 polymorphism in high voltage gel electrophoresis depending on agarose quality investigation of rare complement c 3 variants in behring agarose ags 082. Zeitschrift fuer Rechtsmedizin 77(4): 289-293

Different batches of agarose may lead to migration differences in the demonstration of C3 [third component of the human complement system] polymorphism in high-voltage gel electrophoresis. A particular batch (i.e., AGS 082) which caused 2 basic phenomena concerning mobility and electrophoretic position of C3 phenotypes was studied. C3 variants migrated to the cathode, apparently due to a positive net charge of the protein caused by this agarose batch. The relative mobility of slow and fast bands was virtually reversed. Previously obtained results were controlled and verified by further investigations on rare C3 variants. The possible causes of the net charge differences of the C3 proteins in various agarose batches were discussed.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 005151196

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Reuter, W., 1976: Differences in the demonstration of C3 polymorphism in high-voltage gel electrophoresis depending on agarose quality. Investigations of rare C3 variants in Behring agarose AGS 082. Different batches of agarose may lead to migration differences in the demonstration of C3 polymorphism in high-voltage gel electrophoresis. We studied a particular batch (i.e. AGS 082) which caused two basic phenomena concerning mobility and elect...

Teisberg P., 1970: High voltage agarose gel electrophoresis in the study of 3rd component of complement polymorphism. Vox Sanguinis 19(1): 47-56

Johnson, E.; Eskeland, T., 1983: Complement (C3)-receptor-mediated phagocytosis of agarose beads by mouse macrophages. II. Extracellular activation of macrophage-derived complement on agarose via the alternative pathway. Phagocytosis of agarose beads by macrophages cultured under serum-free conditions was studied. 48 h was needed before a plateau in the uptake was reached. The ingested agarose beads were coated extracellularly with macrophage-derived protein befor...

Johnson, E.; Eskeland, T., 1983: Complement c 3 receptor mediated phagocytosis of agarose beads by mouse macrophages 2. extracellular activation of macrophage derived complement on agarose via the alternative pathway. Phagocytosis of agarose beads by macrophages cultured under serum-free conditions was studied; 48 h was needed before a plateau in the uptake was reached. The ingested agarose beads were coated extracellularly with macrophage-derived protein befor...

Alper C.A.; Propp R.P., 1968: Genetic polymorphism of the 3rd component of human complement inst agarose electrophoresis. Journal of Clinical Endocrinology & Metabolism 47(9): 2181-2191

Teisberg, P., 1970: High voltage agarose gel electrophoresis in the study of C 3 polymorphism. Vox Sanguinis 19(1): 47-56

Rittner, C.H.; Rittner, B., 1975: Transferrin variants designated by their relative mobilities in high-voltage agarose gel electrophoresis. A unified nomenclature for the designation of transferrin variants, based on their relative mobilities in a standardized high-voltage agarose gel electrophoresis, is proposed. This is aimed at improving terminology of Tf variant typing results wit...

Kühnl, P.; Nowicki, L.; Spielmann, W., 1974: Investigations on the polymorphism of red cell esterase D by high voltage agarose gel electrophoresis. Zeitschrift für Rechtsmedizin. Journal of Legal Medicine 75(3): 179-182

Köster, B.; Leupold, H.; Mauff, G., 1975: Esterase D polymorphism: high-voltage agarose-gel electrophoresis and distribution of phenotypes in different European populations. Esterase D phenotypes were determined in 1082 non-related individuals from the western region of Germany by agarose-gel electrophoresis. Gene frequencies were compared with previous data and all European populations studied so fare agreed with the...

Mauff G.; Freis H.; Potrafki B.G.; Hummel K.; Pulverer G., 1974: Problems in the determination of rare variants in complement c 3 polymorphism as demonstrated in 2 families with complement c 3 variants f o 65 and f o 5. Humangenetik 22(3): 211-220