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Differences in the demonstration of complement c 3 polymorphism in high voltage gel electrophoresis depending on agarose quality investigation of rare complement c 3 variants in behring agarose ags 082






Zeitschrift fuer Rechtsmedizin 77(4): 289-293

Differences in the demonstration of complement c 3 polymorphism in high voltage gel electrophoresis depending on agarose quality investigation of rare complement c 3 variants in behring agarose ags 082

Different batches of agarose may lead to migration differences in the demonstration of C3 [third component of the human complement system] polymorphism in high-voltage gel electrophoresis. A particular batch (i.e., AGS 082) which caused 2 basic phenomena concerning mobility and electrophoretic position of C3 phenotypes was studied. C3 variants migrated to the cathode, apparently due to a positive net charge of the protein caused by this agarose batch. The relative mobility of slow and fast bands was virtually reversed. Previously obtained results were controlled and verified by further investigations on rare C3 variants. The possible causes of the net charge differences of the C3 proteins in various agarose batches were discussed.


Accession: 005151196



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