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Differences in the distribution of urea and creatinine between red cells and plasma in normal and azotemic blood as assessed by autoanalyzer and manual chemical methods


, : Differences in the distribution of urea and creatinine between red cells and plasma in normal and azotemic blood as assessed by autoanalyzer and manual chemical methods. International Urology and Nephrology 10(1): 59-64

Urea and creatinine may bind to red blood cell [RBC] constituents. Whole blood and plasma concentrations of urea and creatinine were compared using autoanalyzer techniques (that require solutes to pass through a dialysis membrane) and manual techniques (with no dialysis membrane). Blood samples from 11 normal and 10 azotemic subjects were studied. Concentration differences between plasma water and RBC water for these solutes were significant in normals with standard manual but not with autoanalyzer methods. Accordingly, RBC water concentrations in normals were greater with manual methods than with autoanalyzer methods. In azotemics, differences between RBC and plasma water concentrations and differences in RBC water concentrations between methods were proportionately less. Accumulation of these solutes in the RBC in azotemia was predominantly in the freely diffusable form. Any chemical interference of RBC proteins and/or solute binding to RBC constituents yields significant discrepancies between manual and autoanalyzer methods only at lower BUN [blood urea nitrogen] and creatinine concentrations.

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Accession: 005151214

PMID: 649287

DOI: 10.1007/bf02082794

PDF Full Text: Differences in the distribution of urea and creatinine between red cells and plasma in normal and azotemic blood as assessed by autoanalyzer and manual chemical methods


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