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Differences in the DNA restriction patterns between sheep with HbA and HbB

Animal Genetics 18(3): 241-247

Differences in the DNA restriction patterns between sheep with HbA and HbB

DNA samples obtained from sheep homozygous for HbA, for HbB and heterozygous were subjected to Southern blot analysis using a goat .beta.F and a rabbit .beta.1-globin gene as probes. Sheep homozygous for HbA show a different restriction pattern from that of sheep homozygous for HbB with each of the used endonucleases. The DNAs from moufflon and sheep homozygous for HbA show indistinguishable restriction patterns with some endonucleases. By means of double digestions it has been possible to construct restriction maps of the .beta.B and .beta.C genes and to confirm the absence of the .beta.C gene in HbB sheep.

Accession: 005151216

PMID: 2821850

DOI: 10.1111/j.1365-2052.1987.tb00764.x

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