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Differences in the electric birefringence of spectrin dimers and tetramers as shown by the fast reversing electric pulse method


, : Differences in the electric birefringence of spectrin dimers and tetramers as shown by the fast reversing electric pulse method. Biophysical Chemistry 16(3): 193-198

The electric birefringence of purified [human blood] spectrin was examined in medium of low ionic strength at 20.degree. C and for electric fields < 4 .times. 104 V m-1, using the reversing electric pulse method. This technique allows study of the permanent and induced dipole electric moment of macromolecules more easily than in measurements using only rectangular pulses. Spectrin heterodimers and heterotetramers have different electrooptical properties. The relaxation time of the tetramer (7 .mu.s) is significantly longer than that of the dimer (4.5 .mu.s). Tetramers and dimers have also different polarizability parameters.

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Accession: 005151258

PMID: 7171714

DOI: 10.1016/0301-4622(82)87002-6

PDF Full Text: Differences in the electric birefringence of spectrin dimers and tetramers as shown by the fast reversing electric pulse method


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