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Differences in the infectivity of Babesia incubated in plasma and serum and the role of glucose in prolonging viability


International Journal for Parasitology 6(6): 513-516
Differences in the infectivity of Babesia incubated in plasma and serum and the role of glucose in prolonging viability
B. rodhaini was less infective to mice after incubation in rat serum than in rat plasma. This was explained by lower levels of glucose in serum than in plasma. Both serum and plasma became metabolically depleted of glucose following prolonged contact with clotted and unclotted blood cells, respectively. When glucose concentrations in depleted samples were restored to those in freshly separated samples, infectivities of parasites were similar. This was shown for B. rodhaini and B. argentina. Products of blood cell metabolism produced when separation of plasma or serum was delayed for 24 h, and not have any detrimental effects on the parasites. Average glucose values for plasma from rats and cattle were 153 mg/100 ml and 63 mg/100 ml, respectively, whereas serum and plasma remaining in contact with blood cells contained as little as 2 mg glucose/100 ml. Lactate values were correspondingly low in plasma and high in serum. Fibrinogen and platelets, factors involved in clotting, did not affect infectivity of B. rodhaini or B. argentina. The relevance of these findings to living babesial vaccines in which plasma- and serum-based diluents may be used is discussed.


Accession: 005151325

PMID: 1087630

DOI: 10.1016/0020-7519(76)90090-4



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