+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Differences in the kinetics of axonal transport for individual lipid classes in rat sciatic nerve

, : Differences in the kinetics of axonal transport for individual lipid classes in rat sciatic nerve. Journal of Neurochemistry 40(2): 555-562

Lipid precursors ([2-3H]glycerol for phospholipids and [3H]acetate for cholesterol) were injected into the L[lumbar]-5 dorsal root ganglion of adult rats. At various times, animals were killed, the ganglion and consecutive 5-mm segments of sciatic nerve were dissected, and lipids were extracted and analyzed by TLC. Individual lipid classes exhibited markedly different transport patterns. The crest of radioactive phosphatidylcholine moved as a sharply defined front at .apprx. 300 mm/day, with a relatively flat plateau behind the moving crest. Although some radioactive phosphatidylethanolamine also moved at the same rate, the crest was continually attenuated as it moved so that a gradient of radioactive phosphatidylethanolamine along the axon was maintained for several days. Trasported diphosphatidylglycerol exhibited a defined crest, as did phosphatidylcholine, but moved at about half the rate. Labeled cholesterol was transported at a rapid rate similar to that for phosphatidylcholine and phosphatidylethanolamine, but like phosphatidylethanolamine, the initial moving crest of radioactivity was continually attenuated. Relative to the phospholipids, cholesterol showed a more prolonged period of accumulation in the axons and was more metabolically stable. Most labeled phosphatidylcholine, phosphatidylethanolamine and cholesterol is probably transported in similar (or the same) rapidly moving membranous particles. Once incorporated into these particles, molecules of phosphatidylcholine tend to remain associated with them during transport. Molecules of phosphatidylethanolamine and cholesterol in these transported particles exchange extensively with unlabeled molecules in stationary axonal structures. Diphosphatidylglycerol, localized in a specialized organelle, the mitochondrion, is transported at slower rate than other phospholipids and does not exchange with other structures.

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 005151352

PMID: 6185640

DOI: 10.1111/j.1471-4159.1983.tb11318.x

PDF Full Text: Differences in the kinetics of axonal transport for individual lipid classes in rat sciatic nerve

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Tatum, W.M.; Blackburn, R.D., 1962: Preliminary study of the effects of diquat on the natural bottom fauna and plankton in two subtropical ponds. In trials in which diquat was applied in ponds at 0.5 ppmw., plankton appeared to be adversely affected immediately after treatment, but recovered rapidly. Diquat showed a subtle chronic toxicity to Oligochaeta, but had a variable effect on Chiron...

Talini, D.; Petrozzino, M.; Benvenuti, A.; Innocenti, G.; Carletti, A.; Macchioni, P.; Taccola, M.; Paggiaro, P.L.; Giuntini, C., 1995: Risk factors for asthma in furniture workers. European Respiratory Journal 8(SUPPL 19): 272S

Jeong, J.Min.; Hong, M.Kyung.; Chang, Y.Soo.; Lee, Y-Sang.; Kim, Y.Joo.; Cheon, G.Jeong.; Lee, D.Soo.; Chung, J-Key.; Lee, M.Chul., 2008: Preparation of a promising angiogenesis PET imaging agent: 68Ga-labeled c(RGDyK)-isothiocyanatobenzyl-1,4,7-triazacyclononane-1,4,7-triacetic acid and feasibility studies in mice. Arg-Gly-Asp (RGD) derivatives have been labeled with various radioisotopes for the imaging of angiogenesis in ischemic tissue, in which alpha(v)beta(3) integrin plays an important role. In this study, cyclic Arg-Gly-Asp-D-Tyr-Lys [c(RGDyK)] was co...

Li, H.; Roux, S.J.; Roux, S.J., 1992: Casein kinase II protein kinase is bound to lamina-matrix and phosphorylates lamin-like protein in isolated pea nuclei. A casein kinase II (CK II)-like protein kinase was identified and partially isolated from a purified envelope-matrix fraction of pea (Pisum sativum L.) nuclei. When [gamma-32P]ATP was directly added to the envelope-matrix preparation, the three mo...

Jackson, P.L., 1979: Congenital heart disease. Digoxin therapy at home: keeping the child safe. Mcn. American Journal of Maternal Child Nursing 4(2): 105-109

Yoda, K.; Naito, Y.; Sato, K.; Takahashi, A.; Nishikawa, J.; Ropert Coudert, Y.; Kurita, M.; L.M.ho, Y., 2001: A new technique for monitoring the behaviour of free-ranging Adelie penguins. Measurement of the time allocation of penguins at sea has been a major goal of researchers in recent years. Until now, however, no equipment has been available that would allow measurement of the aquatic and terrestrial behaviour of an Antarctic p...

Fowler, D.; Wiselogel, A., 1993: Pollen polymixes. Agriculture handbook: (698) 79-82

Alten, F.; Niemann, A., 1959: Relationships between stage of maturity, phosphorus metabolism and potassium nutrition.. Analysis of the sap of sugar-beet roots grown under differential fertilizing in pots showed gluta-mine contents to increase sharply with increasing supply of N, and to decrease with increasing supply of K. An increasing K supply promotes the uptak...

Allen, C.M.; Chakravarthy, B.; Morley, P.; Whitfield, J.F.; Willick, G.E., 2002: HPTH- and hPTH- NH2 prevent phosphate from killing MC3T3-E1 preosteoblasts and HKRKB7 pig kidney cells. Journal of Bone & Mineral Research 17(Suppl 1): S390, September

Gabriely, I.; Barzilai, N., 2003: Surgical removal of visceral adipose tissue: effects on insulin action. Many studies have demonstrated that excess of visceral fat has deleterious effects on insulin action. Mainly, it has been shown to be associated with a decrease in hepatic and peripheral insulin sensitivity, which results in a clinical condition a...