EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Differences in the mode of action of antimycin and 2 nonyl 4 hydroxy quinoline n oxide on oxidation reduction of mitochondrial cytochromes b


Biokhimiya 48(9): 1456-1462
Differences in the mode of action of antimycin and 2 nonyl 4 hydroxy quinoline n oxide on oxidation reduction of mitochondrial cytochromes b
The differences in the effects of 2 similar electron transfer inhibitors, antimycin and 2-nonyl-4-hydroxyquinoline N-oxide (HOQNO) on the reactions of cytochromes b are described. In the cyanide-inhibited submitochondrial particles of bovine heart, HOQNO strongly stimulates cytochrome b reduction by ascorbate in the presence of redox mediators, e.g., N,N,N'N'-tetramethylparaphenylene diamine, 2,6-dichlorophenolindophenol, diaminodurol and phenazine methosulfate; this effect can be reversed by antimycin. Addition of both inhibitors to the submitochondrial particles aerobically equilibrated with succinate/fumarate redox buffer at E [potential] = + 54 mV in the presence of cyanide results in a similar reduction of cytochromes b, which in the case of antimycin is readily reversed by phenazine methosulfate but is resistant to this redox mediator in the presence of HOQNO. The latter causes additional reduction of cytochrome b562 in an argon atmosphere, the effect being reversed by antimycin. In the presence of HOQNO the anaerobic redox titration curve of cytochrome b562 is shifted towards a high potential region by 20-30 mV. An additional reduction of cytochromes b induced by HOQNO may be due to the superposition of 2 effects, i.e. extra-reduction of cytochromes b566 and b562 requiring O2 and a true positive shift of E'0 [midpoint potential] of cytochrome b562.


Accession: 005151409



Related references

Differences in the action of antimycin and 2-nonyl-4-hydroxyquinoline N-oxide on oxidation-reduction of mitochondrial cytochromes b. Biokhimiia 48(9): 1456-1462, 1983

Effects of antimycin a and 2 heptyl 4 hydroxy quinoline n oxide on the b cytochromes associated with beef heart sub mitochondrial membrane. Federation Proceedings: 447, 1972

2-n-nonyl-4-hydroxy[3-3H]quinoline N-oxide: interaction with the mitochondrial membrane. European Journal of Biochemistry 114(1): 153-157, 1981

Tritium labeled 2 n nonyl 4 hydroxy quinoline n oxide interaction with the mitochondrial membrane. European Journal of Biochemistry 114(1): 153-158, 1981

Interaction of tritium labeled 3 2 n nonyl 4 hydroxy quinoline n oxide with sub mitochondrial particles of bovine heart 3. correlation between the tritium labeled nonyl link and the inhibition of respiratory activity. Bollettino Societa Italiana Biologia Sperimentale 55(23): 2419-2424, 1979

The inhibitor tritium labeled 2 n nonyl 4 hydroxy quinoline n oxide as a ligand to the mitochondrial membrane. Italian Journal of Biochemistry (English Edition) 29(6): 467-468, 1980

Interaction of tritium labeled 3 2 n nonyl 4 hydroxy quinoline n oxide with sub mitochondrial particles of bovine heart 1. inhibition of respiratory activity. Bollettino Societa Italiana Biologia Sperimentale 55(23): 2406-2411, 1979

Interaction of tritium labeled 3 2 n nonyl 4 hydroxy quinoline n oxide with sub mitochondrial particles of bovine heart 2. determination of the binding sites. Bollettino Societa Italiana Biologia Sperimentale 55(23): 2412-2418, 1979

The effect of antimycin on the b-cytochromes of plant mitochondria: Oxidation-reduction behavior of cytochromes b-566. Biochemical and biophysical research communications: 52 (3) 703-711, 1973

The inhibitory effect of 2 heptyl 4 hydroxy quinoline n oxide compared with extra reduction and binding in the wild type and in an antimycin resistant mutant ant r 8 in schizosaccharomyces pombe. Bandlow W Et Al (Ed ) Genetics, Biogenesis And Bioenergetics Of Mitochondria Proceedings Of A Symposium Munich, West Germany, Sept 11-13, 1975 Xvi+418p Illus Walter De Gruyter: Berlin, West Germany; New York, N Y , Usa Isbn 3-11-006865-6 399-407, 1976