EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Differences in the rates of internalization of iodine 125 labeled human chorionic gonadotropin luteinizing hormone and epidermal growth factor by ovine luteal cells


, : Differences in the rates of internalization of iodine 125 labeled human chorionic gonadotropin luteinizing hormone and epidermal growth factor by ovine luteal cells. Endocrinology 113(1): 259-264

The rate of internalization and degradation of radioiodinated hCG [human chorionic gonadotropin], ovine LH [luteinizing hormone] (oLH), human LH (hLH) and mouse epidermal growth factor (mEGF) was studied in suspensions of ovine luteal cells. Hormone bound to the plasma membrane was quantified by treating the cells with acidic buffer (pH 3.0) and the radioactivity removed from the receptor was measured. The radioactivity remaining in the cell pellet was considered to have been internalized. The extent of degradation of the radioactive hormones was determined by subjecting the radioactivity that was released into the medium during the incubation periods to precipitation with 20% trichloroacetic acid. The non-precipitable radioactivity was considered to have been degraded. The required time for loss of half of the radioactivity that had been bound to the cell membrane at time zero was 22.8 .+-. 2.3 h for hCG, 23.3 .+-. 1.1 h for asialo-hCG, 15.1 .+-. 1.4 h for hLH, 0.4 .+-. 0.2 h for oLH and 0.3 .+-. 0.1 h for mEGF. The quantities of radioactivity that had been internalized (intracellular plus degraded) were greater at earlier times (15-120 min) for oLH or mEGF than for hCG, asialo-hCG or hLH. Apparently ligands bound to different receptors on ovine luteal cells are internalized and degraded at different rates by the same cell, and that ligands that bind to the same receptor (hCG, hLH and oLH) can also be internalized and degraded at very different rates.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 005151521

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Dor I., 1985: Long lasting effect of dilution on the cell volume motility division rate and vertical distribution of dunaliella parva. The effects of dilution leading to loss of buoyancy and sinking of Dunaliella were studied. Dilution causes an immediate cell swelling which changes cell shape from elongated to more spherical, and is connected with a cessation of flagellar moveme...

Van-Den-Berghe, C.; Sota, P.; Mujawayezu, A., 1994: Study on mineral complementation in the composting process in farmers conditions of Mugamba, Burundi (in French). In this study which is a continuation of the research done on farmers fields on potatoes in the natural region of Mugamba (9), three methods of composting have been tested: the composting without fertilizer addition, the one with addition of 5 kg...

Mulroy, Michael F.; Mitchell, Blake, 2002: Unsolicited paresthesias with nerve stimulator Case reports of four patients. Anesthesia & Analgesia 95(3): 762-763, September

Gregory, P. J.; Ingram, J. S. I., 2003: Global environmental change and future crop production. Global environmental change (GEC) is a consequence of a range of human activities and includes elements such as increasing concentrations of gases in the atmosphere, climate variation and change, rising sea level, loss of biodiversity, and changes...

Ezrin-Waters, C.; Seeman, P., 1977: Tolerance to haloperidol catalepsy. Haloperidol (0.75 and 1.5 mg/kg orally) was administered daily for 16 days to male Wistar rats. The animals received an acute injection of haloperidol (0.5-2.0 mg/kg i.p. or 1.0-4.0 mg/kg orally) and catalepsy was measured. After 16 days on halope...

DeLoach, C. J., 1975: Identification and biological notes on the species of Neochetina that attack Pontederiaceae in Argentina (Coleoptera: Curculionidae: Bagoini). This paper on the weevil Neochetina on aquatic plants of the family Pontederiaceae in Argentina includes notes on the biology of 4 species (including some that are under consideration for the control of Eichhornia crassipes) and a key for their se...

Zelzer, Elazar; Glotzer, Donald J.; Hartmann, Christine; Thomas, David; Fukai, Naomi; Soker, Shay; Olsen, Bjorn R., 2001: Tissue specific regulation of VEGF expression during bone development requires Cbfa1/Runx2. Vascular endothelial growth factor (VEGF) is a critical regulator of angiogenesis during development, but little is known about the factors that control its expression. We provide the first example of tissue specific loss of VEGF expression as a r...

Cvoro, Aleksandra; Paruthiyil, Sreenivasan; Jones, Jeremy, O.; Tzagarakis-Foster, Christina; Clegg, Nicola, J.; Tatomer, Deirdre; Medina, Roanna, T.; Tagliaferri, Mary; Schaufele, Fred; Scanlan, Thomas, S.; Diamond, Marc, I.; Cohen, Isaac; Leitman, Dale, C., 2007: Selective activation of estrogen receptor-beta transcriptional pathways by an herbal extract. Novel estrogenic therapies are needed that ameliorate menopausal symptoms and have the bone-sparing effects of endogenous estrogens but do not promote breast or uterine cancer. Recent evidence suggests that selective activation of the estrogen re...

Andreeva, V. A. : Romanova, S. A.; Ivanova, T. P., 1977: Activity of oxidation-reduction enzymes in leaves of D. stramonium L. infected by different strains of potato virus X. Peroxidase and polyphenol oxidase activity in infected leaves of D[atura] stramonium was higher with more aggressive strs. of the virus.

Szentmihalyi, K.; Hajdu, M.; Fodor, J.; Kotai, L.; Blazovics, A.; Somogyi, A.; Then, M., 2006: In vitro study of elements in herbal remedies. Decreased glucose tolerance is a first sign of diabetes mellitus and therefore rigorous control must be taken in carbohydrate and lipid metabolisms. Herbal remedies (lyophilized extracts of Myrtilli folium and Phase-oli fructus side seminibus (L1...