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Differences in the response of several cell types to inhibition of surface receptor mobility by local concanavalin A binding


Experimental Cell Research 136(1): 189-201
Differences in the response of several cell types to inhibition of surface receptor mobility by local concanavalin A binding
Concanavalin A (conA) modulates the lateral mobility of cell surface receptors differently on different cell types. This was demonstrated by using fluorescence photobleaching recovery (FPR) to measure the inhibition of the lateral mobility of conA receptors, localizing binding of conA on lymphocytes, fibroblasts, and macrophages. On mouse spleen lymphocytes, binding of conA platelets above a threshold coverage (.apprx. 12% of the upper cell-surface area) reduced the diffusion coefficient of mobile TMR-SconA[tetramethylrhodamine-succinyl concanavalin A]-receptor complexes from 3.0 .times. 10-10 cm2/s-0.6 .times. 10-10 cm2/s (a 5-fold decrease); the fraction of mobile receptors was concomitantly reduced from 0.4-0.11. Below the threshold occupancy, no effect on either parameter was detected. On [mouse fibroblast] 3T3 cells, a qualitatively similar threshold phenomenon was observed: coverage of > 9% of the upper cell surface by conA [human blood] platelets induced a 3-fold reduction in the diffusion coefficient of TMR-SconA-receptor complexes from 5 .times. 10-10 cm2/s-1.7 .times. 10-10 cm2/s. No effect on the mobile fraction (.apprx. 0.4) was observed. Neither the diffusion coefficient nor the mobile fraction of TMR-SconA-receptor complexes on mouse peritoneal macrophages (both resident and thioglycolate-stimulated), or on the mouse macrophage cell line P388D1, was affected by the binding of conA platelets in amounts covering > 50% of the upper cell surface (.apprx. 4.6 .times. 10-10 cm2/s and 0.5 for the diffusion coefficient and mobile fraction, respectively). These differences are correlated to the different cytoskeletal functions of the various cell types studied, and are discussed regarding the mechanism of the conA-induced modulation.


Accession: 005151563

PMID: 7297611



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