EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Differences in the uptake of transferrin bound plutonium 239 and iron 59 into multicellular spheroids of hepatocytes from adult male rats


International Journal of Radiation Biology & Related Studies in Physics Chemistry & Medicine 52(6): 883-892
Differences in the uptake of transferrin bound plutonium 239 and iron 59 into multicellular spheroids of hepatocytes from adult male rats
Hepatocytes were cultured as monolayers and multicellular spheroids, respectively. The uptake of both transferrin-bound metals, iron and plutonium, differed significantly between these two culture systems. The uptake into the multicellular spheroids for plutonium was about 30 times greater, and for iron about 4 times greater, than in monolayer-cultured hepatocytes, which is not a consequence of proliferation and/or de-differentiation of the hepatocytes in the multicellular spheroid culture system. A comparison of the iron and platonium uptake showed that plutonium was delivered to the cells to an 8-fold greater extent than iron if the hepatocytes were cultured as spheroids. Additionally, the binding of plutonium was not inhibited by preincubation of the spheroids with the iron-transferrin complex. Therefore, we propose that there are two different binding sites for iron and plutonium on hepatocytes membranes.


Accession: 005151664



Related references

Differences in the uptake of transferrin bound 239Pu and 59Fe into multicellular spheroids of hepatocytes from adult male rats. International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine 52(6): 883-892, 1987

Uptake of metal transferrin complexes into multicellular spheroids of rat hepatocytes. Biological Chemistry Hoppe-Seyler 368(10): 1270, 1987

The role of Hfe in transferrin-bound iron uptake by hepatocytes. Hepatology 47(5): 1737-1744, 2008

Non-transferrin-bound iron uptake by isolated rat hepatocytes. Gastroenterology 106(4 SUPPL ): A864, 1994

Characterization of non-transferrin-bound iron (ferric citrate) uptake by rat hepatocytes in culture. Biochimica et Biophysica Acta 1380(1): 21-30, 1998

Non-transferrin-bound iron uptake via cell surface DMT1 on human hepatocytes. Blood 102(11): 759a, November 16, 2003

The influence of gallium and other metal ions on the uptake of non-transferrin-bound iron by rat hepatocytes. Biochimie 88(6): 645-650, 2006

Characterisation of non-transferrin-bound iron (ferric citrate) uptake by rat hepatocytes in culture. Biochimica et Biophysica Acta 1380(1): 21-30, 1998

Aluminum stimulates uptake of non-transferrin bound iron and transferrin bound iron in human glial cells. Toxicology and Applied Pharmacology 220(3): 349-356, 2007

Uptake of iron from N-terminal half-transferrin by isolated rat hepatocytes. Evidence of transferrin-receptor-independent iron uptake. European Journal of Biochemistry 232(1): 129-133, 1995